Biotechnology Set 1

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This set of Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on Biotechnology Set 1

Q1 | Restriction enzymes were discovered by
  • Smith and Nathans
  • Alexander Fleming
  • Berg
  • None
Answer: Smith and Nathans
Q2 | Bacteria protect themselves from viruses by fragmenting viral DNA with
  • Ligase
  • Endonuclease
  • Exonuclease
  • Gyrase
Answer: Endonuclease
Q3 | Klenow fragment is derived from
  • DNA Ligase
  • DNA Pol-I
  • DNA Pol-II
  • Reverse Transcriptase
Answer: DNA Pol-I
Q4 | Southern blotting is
  • Attachment of probes to DNA fragments
  • Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
  • Comparison of DNA fragments to two sources
  • Transfer of DNA fragments to electrophoretic gel from cellulose membrane
Answer: Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
Q5 | ELISA is
  • Using radiolabelled second antibody
  • Usage of RBCs
  • Using complement-mediated cell lysis
  • Addition of substrate that is converted into a coloured end product
Answer: Addition of substrate that is converted into a coloured end product
Q6 | The Golden Rice variety is rich in
  • Vitamin C
  • ?-carotene and ferritin
  • Biotin
  • Lysine
Answer: ?-carotene and ferritin
Q7 | The DNA fragments have sticky ends due to
  • Endonuclease
  • Unpaired bases
  • Calcium ions
  • Free methylation
Answer: Unpaired bases
Q8 | Plasmids are used as cloning vectors for which of the following reasons?
  • Can be multiplied in culture
  • Self-replication in bacterial cells
  • Can be multiplied in laboratories with the help of enzymes
  • Replicate freely outside bacterial cells
Answer: Self-replication in bacterial cells
Q9 | The human genome project was launched in the year
  • 1980
  • 1973
  • 1990
  • 1989
Answer: 1990
Q10 | The vaccines prepared through recombinant DNA technology are
  • Third generation vaccines
  • First-generation vaccines
  • Second-generation vaccines
  • None
Answer: Third generation vaccines
Q11 | Which is a genetically modified crop?
  • Bt-cotton
  • Bt-brinjal
  • Golden rice
  • All
Answer: All
Q12 | PCR technique was invented by
  • Karry Mullis
  • Boyer
  • Sanger
  • Cohn
Answer: Karry Mullis
Q13 | The first transgenic plant to be produced is
  • Brinjal
  • Tobacco
  • Rice
  • Cotton
Answer: Tobacco
Q14 | RNA interference helps in
  • Cell proliferation
  • Micropropagation
  • Cell defence
  • Cell differentiation
Answer: Cell defence
Q15 | Which of the following is the quality of improved transgenic basmati rice?
  • Gives high yield but no characteristic aroma
  • Gives high yield and is rich in vitamin A
  • Does not require chemical fertilizers and growth hormones
  • Resistant to insects and diseases
Answer: Gives high yield and is rich in vitamin A
Q16 | The first clinical application of gene therapy over a 4 year old girl was for
  • Adenosine deaminase deficiency
  • Adenosine deficiency
  • Growth deficiency
  • Adenine deficiency
Answer: Adenosine deaminase deficiency
Q17 | Excision and insertion of a gene is called
  • Biotechnology
  • Genetic engineering
  • Cytogenetics
  • Gene therapy
Answer: Genetic engineering
Q18 | The expression of a transgene in the target tissue is identified by a
  • Transgene
  • Promoter
  • Enhancer
  • Reporter
Answer: Reporter
Q19 | ———– is used as a vector for cloning into higher organisms
  • Retrovirus
  • Baculovirus
  • Salmonella typhimurium
  • Rhizopus nigricans
Answer: Retrovirus
Q20 | Which bacterium is used in the production of insulin by genetic engineering?
  • Saccharomyces
  • Rhizobium
  • Escherichia
  • Mycobacterium
Answer: Escherichia
Q21 | The Taq polymerase enzyme is obtained from
  • Thermus aquaticus
  • Thiobacillus ferrooxidans
  • Bacillus subtilis
  • Pseudomonas subtilis
Answer: Thermus aquaticus
Q22 | Which of the following is an endonuclease?
  • DNase I
  • Hind II
  • Protease
  • RNase restriction
Answer: Hind II
Q23 | Which of the following restriction enzymes produce blunt ends?
  • Hind III
  • ho
  • Eco RV
  • Sal I
Answer: Eco RV
Q24 | Which of the following is not a component of downstream processing?
  • Expression
  • Preservation
  • Purification
  • Separation
Answer: Expression
Q25 | A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form arecombinant plasmid using
  • Taq polymerase
  • Polymerase III
  • Ligase
  • Eco RI
Answer: Ligase
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