Biotechnology Principles and Processes Set 2 MCQs

Q1 | Particle bombardment technique is also known as .....

Lipofection

Electroporation

Biolistic

Micro injection

Q2 | Which enzyme is used to break the membrane to relase plant DNA ?

Lysozyme

Chitinase

Cellulose

All the above

Q3 | Which enzyne is used to break the membrane to relase animal DNA ?

Lysozyme

chitinase

Celluose

All the above

Q4 | Which is the first step in the process recombinant DNA technology ?

denaturing of DNA

Annealing of DNA

Isolation of Donor DNA

Down streaming

Q5 | Which primers are used in annealing during amplification of gene ?

Reverse primers

Forward primers

Oligo nucleotide primer

Internal primers

Q6 | Which of the following is related with genetic engineering ?

Breeding

somatic hybridization

mutation

Transgenic

Q7 | What is C - DNA ?

circular DNA

Cloned DNA

DNA produced from reverse transcription of RNA

Cytoplasmic DNA

Q8 | Which of the bollowing statement is incorrect ?

cosmid contains gene coding for viral protein

cosmid relpicates like plasmids

cosmid has antibioticresistant marker gene

cos sit has 12 bases helping to join complete genome to make it circular

Q9 | The genetic recombinants obtained by in sertion of plasmid into 1 phage genome is called

cosmid

plasmid

phagmid

foreign DNA

Q10 | TATAATG sequnce near the RNA start point of phokaryotic promoter is......

NICKS

DNA marker

pallindrome

pribnow box

Q11 | Exonucleases cleaving nucleotides one at a time from the end of polynucleotide chain are.

Specific for 5’ end of RNA strand

specific for 3’ end of RNA strand

specific for both 5’ and 3’ ends of nucleotide strand

Non- specific for 5’ and 3’ ends of nucleotide

Q12 | Genes that are involved in turning on or off the transcription of a set of structural genes are called

Polymorphic genes

operator gene

Rebundant gene

Regulatory gene

Q13 | Enzymes used in PCR is ....

taq polymerase

gyrase

transcriptase

hexokinase

Q14 | What are structure labelled A & B respectively

EcoRv restriction endonuclease and E coRv ligase

EcoRv ligase and EcoRv nuclease and EcoRvmethlase

Eco–– Rv restriction endo EcoRv methylase

EcoRv Polymerase and EcoRv methylase

Q15 | Can you pick up from the figure how bacteria protects its own genone from degradation by restreiction endonuclease ?

site specific coupling

site specific oxidizing

site specific methylases

Q16 | This is figure of plasmid P BR322 Identiby what represented by A, B, and C

A TcR, B ApR and C EcorI

A TcR, B EcoRI and C ApR

A - EcorI, B ApR and C TcR

A ApR, B TcR and C EcoRz

Q17 | These are important set of enzymes used in biotechnology Match them with exact roleP Taq DNa Polymerase (i) cutting single stranded part of DNAQ S I nuclease (ii) LigaseR Restriction endo nuclease (iii) Thermostable enzymeS mole cular glue (iv) cutting pallindromic sequences(v) union of pallindromic sequencesP Q R S

(iii) (iv) (i) (ii)

(iii) (v) (iv) (ii)

(iv) (i) (v) (ii)

(iii) (iv) (i) (ii)

Q18 | Match the column I and column IIP Radio active andibody (a) substance that can be constructed in the laboratoryQ Artificial gene (b) substance that can be used to identify colonies of geneticully engineered bacteria that makes particular gene productR Amplification (c) Abnormal enhanced replication of a plasmid many copies of plasmid in each cellS To produce clones (d) A large population of idential cellsT short gun cloning (e) The use of entire array of genes of an organism in order to obtain particular gene productP Q R S T

b a c d e

a c b d e

a c d b e

b c e d a

Q19 | For transformation, micro particles coated with DNA are bombarded with gene gun made up of.

Platinum or Zinc

Silicon or Platinum

Gold or tungsten

Silver or Platinum

Q20 | PCR and Restriction fragment lenth Polymorphism are the methods for. (AIPMT-2012)

genetic transtormation

DNA Sequencing

DNA finger printing

Study of enzymes

Q21 | The linking of antibiotic resistance gene with the plasmid Vector became possible with

DNA ligase

Exonuclease

Endo nuclease

DNA Polymerase

Q22 | Gel electrophoresis is used for--

Isolation of DNA molecule

Cutting of DNA in to fragments

Separation of DNA fragments according to their size

Construction of recombinant DNA by joining with cloning Vector

Q23 | Gentic engineering has been sucessfully used for producing ___ (CBSE-2010)

trangenic models for studying new treatments for Certain cardiac diseases.

transgenic Cow - Rosie which produces high fat milk for making ghee.

animals linke bulies for farm work as they have super power.

transgenic mice for testing safety of polio Vaccine before use in humans.

Q24 | Match the following and choose the correct combination from the option given ... (Karnatak PMT-2005)Column I Column II(a) Escherichia coli 1 - nif gene(b) Rhizobium meliloti 2 - digestion of hydrocarbons of crude oil(c) Bacilius thuringiensis 3 - human insulin production(d) Pseudomonas putida 4 - Biocontrol of fungal disease5 - biodegradable insecticide

A = 3, B = 1, C = 5, D = 4

A = 1, B = 2, C = 3, D = 4,

A = 2, B = 1, C = 3, D = 4

A = 4, B = 3, C = 1, D = 2

Q25 | Find the incorrrect statement

Gene therapy is a genetic engineering technique used to treat disease at molecular level by replacing defective genes with normal genes.

Calcitonin is a medically useful recombinant product in the treatment of intetility

Bt toxin is a Biodegradable insecticide obtained from bacillis thuringiensis

Trichoderma sp. is a biocontrol agent for fungal diseases of plants