Biotechnology Principles and Processes Set 2

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This set of Biology Multiple Choice Questions & Answers (MCQs) focuses on Biotechnology Principles and Processes Set 2

Q1 | Particle bombardment technique is also known as .....
  • Lipofection
  • Electroporation
  • Biolistic
  • Micro injection
Q2 | Which enzyme is used to break the membrane to relase plant DNA ?
  • Lysozyme
  • Chitinase
  • Cellulose
  • All the above
Q3 | Which enzyne is used to break the membrane to relase animal DNA ?
  • Lysozyme
  • chitinase
  • Celluose
  • All the above
Q4 | Which is the first step in the process recombinant DNA technology ?
  • denaturing of DNA
  • Annealing of DNA
  • Isolation of Donor DNA
  • Down streaming
Q5 | Which primers are used in annealing during amplification of gene ?
  • Reverse primers
  • Forward primers
  • Oligo nucleotide primer
  • Internal primers
Q6 | Which of the following is related with genetic engineering ?
  • Breeding
  • somatic hybridization
  • mutation
  • Transgenic
Q7 | What is C - DNA ?
  • circular DNA
  • Cloned DNA
  • DNA produced from reverse transcription of RNA
  • Cytoplasmic DNA
Q8 | Which of the bollowing statement is incorrect ?
  • cosmid contains gene coding for viral protein
  • cosmid relpicates like plasmids
  • cosmid has antibioticresistant marker gene
  • cos sit has 12 bases helping to join complete genome to make it circular
Q9 | The genetic recombinants obtained by in sertion of plasmid into 1 phage genome is called
  • cosmid
  • plasmid
  • phagmid
  • foreign DNA
Q10 | TATAATG sequnce near the RNA start point of phokaryotic promoter is......
  • DNA marker
  • pallindrome
  • pribnow box
Q11 | Exonucleases cleaving nucleotides one at a time from the end of polynucleotide chain are.
  • Specific for 5’ end of RNA strand
  • specific for 3’ end of RNA strand
  • specific for both 5’ and 3’ ends of nucleotide strand
  • Non- specific for 5’ and 3’ ends of nucleotide
Q12 | Genes that are involved in turning on or off the transcription of a set of structural genes are called
  • Polymorphic genes
  • operator gene
  • Rebundant gene
  • Regulatory gene
Q13 | Enzymes used in PCR is ....
  • taq polymerase
  • gyrase
  • transcriptase
  • hexokinase
Q14 | What are structure labelled A & B respectively
  • EcoRv restriction endonuclease and E coRv ligase
  • EcoRv ligase and EcoRv nuclease and EcoRvmethlase
  • Eco–– Rv restriction endo EcoRv methylase
  • EcoRv Polymerase and EcoRv methylase
Q15 | Can you pick up from the figure how bacteria protects its own genone from degradation by restreiction endonuclease ?
  • site specific coupling
  • site specific oxidizing
  • site specific oxidizing
  • site specific methylases
Q16 | This is figure of plasmid P BR322 Identiby what represented by A, B, and C
  • A TcR, B ApR and C EcorI
  • A TcR, B EcoRI and C ApR
  • A - EcorI, B ApR and C TcR
  • A ApR, B TcR and C EcoRz
Q17 | These are important set of enzymes used in biotechnology Match them with exact roleP Taq DNa Polymerase (i) cutting single stranded part of DNAQ S I nuclease (ii) LigaseR Restriction endo nuclease (iii) Thermostable enzymeS mole cular glue (iv) cutting pallindromic sequences(v) union of pallindromic sequencesP Q R S
  • (iii) (iv) (i) (ii)
  • (iii) (v) (iv) (ii)
  • (iv) (i) (v) (ii)
  • (iii) (iv) (i) (ii)
Q18 | Match the column I and column IIP Radio active andibody (a) substance that can be constructed in the laboratoryQ Artificial gene (b) substance that can be used to identify colonies of geneticully engineered bacteria that makes particular gene productR Amplification (c) Abnormal enhanced replication of a plasmid many copies of plasmid in each cellS To produce clones (d) A large population of idential cellsT short gun cloning (e) The use of entire array of genes of an organism in order to obtain particular gene productP Q R S T
  • b a c d e
  • a c b d e
  • a c d b e
  • b c e d a
Q19 | For transformation, micro particles coated with DNA are bombarded with gene gun made up of.
  • Platinum or Zinc
  • Silicon or Platinum
  • Gold or tungsten
  • Silver or Platinum
Q20 | PCR and Restriction fragment lenth Polymorphism are the methods for. (AIPMT-2012)
  • genetic transtormation
  • DNA Sequencing
  • DNA finger printing
  • Study of enzymes
Q21 | The linking of antibiotic resistance gene with the plasmid Vector became possible with
  • DNA ligase
  • Exonuclease
  • Endo nuclease
  • DNA Polymerase
Q22 | Gel electrophoresis is used for--
  • Isolation of DNA molecule
  • Cutting of DNA in to fragments
  • Separation of DNA fragments according to their size
  • Construction of recombinant DNA by joining with cloning Vector
Q23 | Gentic engineering has been sucessfully used for producing ___ (CBSE-2010)
  • trangenic models for studying new treatments for Certain cardiac diseases.
  • transgenic Cow - Rosie which produces high fat milk for making ghee.
  • animals linke bulies for farm work as they have super power.
  • transgenic mice for testing safety of polio Vaccine before use in humans.
Q24 | Match the following and choose the correct combination from the option given ... (Karnatak PMT-2005)Column I Column II(a) Escherichia coli 1 - nif gene(b) Rhizobium meliloti 2 - digestion of hydrocarbons of crude oil(c) Bacilius thuringiensis 3 - human insulin production(d) Pseudomonas putida 4 - Biocontrol of fungal disease5 - biodegradable insecticide
  • A = 3, B = 1, C = 5, D = 4
  • A = 1, B = 2, C = 3, D = 4,
  • A = 2, B = 1, C = 3, D = 4
  • A = 4, B = 3, C = 1, D = 2
Q25 | Find the incorrrect statement
  • Gene therapy is a genetic engineering technique used to treat disease at molecular level by replacing defective genes with normal genes.
  • Calcitonin is a medically useful recombinant product in the treatment of intetility
  • Bt toxin is a Biodegradable insecticide obtained from bacillis thuringiensis
  • Trichoderma sp. is a biocontrol agent for fungal diseases of plants