Campbell Biology, 11th Ed: Final Exam Review Flashcards

When Thomas Hunt Morgan crossed his red-eyed F? generation flies to
each other, the F? generation included both red- and white-eyed flies.
Remarkably, all the white-eyed flies were male. What was the
explanation for this result? A) The gene involved is on
the Y chromosome. B) The gene involved is on the X chromosome.
C) The gene involved is on an autosome, but only in males.
D) Other male-specific factors influence eye color in flies.
E) Other female-specific factors influence eye color in flies.

B) The gene involved is on the X chromosome.

Which of the following is the meaning of the chromosome theory of
inheritance as expressed in the early 20th century? A)
Individuals inherit particular chromosomes attached to genes.
B) Mendelian genes are at specific loci on the chromosome and in
turn segregate during meiosis.
C) No more than a single pair of chromosomes can be found in a
healthy normal cell.
D) Natural selection acts on certain chromosome arrays rather
than on genes.

B) Mendelian genes are at specific loci on the chromosome and in turn
segregate during meiosis.

Males are more often affected by sex-linked traits than females
because A) male hormones such as testosterone often
alter the effects of mutations on the X chromosome.
B) female hormones such as estrogen often compensate for the
effects of mutations on the X chromosome.
C) X chromosomes in males generally have more mutations than X
chromosomes in females.
D) males are hemizygous for the X chromosome.
E) mutations on the Y chromosome often worsen the effects of
X-linked mutations.

D) males are hemizygous for the X chromosome.

SRY is best described in which of the following ways?
A) a gene present on the X chromosome that triggers female development
B) an autosomal gene that is required for the expression of genes
on the Y chromosome
C) a gene region present on the Y chromosome that triggers male development
D) an autosomal gene that is required for the expression of genes
on the X chromosome
E) a gene required for development, and males or females lacking
the gene do not survive past early childhood

C) a gene region present on the Y chromosome that triggers male development

When Thomas Hunt Morgan crossed his red-eyed F? generation flies to
each other, the F? generation included both red- and white-eyed flies.
Remarkably, all the white-eyed flies were male. What was the
explanation for this result? A) The gene involved is on
the Y chromosome. B) The gene involved is on the X chromosome.
C) The gene involved is on an autosome, but only in males.
D) Other male-specific factors influence eye color in flies.
E) Other female-specific factors influence eye color in flies.

B) The gene involved is on the X chromosome.

Red-green color blindness is a sex-linked recessive trait in humans.
Two people with normal color vision have a color-blind son. What are
the genotypes of the parents?
A) XnXn and XnY
B) XnXn and XNY
C) XNXN and XnY
D) XNXN and XNY
E) XNXn and XNY

E) XNXn and XNY

Cinnabar eyes is a sex-linked recessive characteristic in fruit
flies. If a female having cinnabar eyes is crossed with a wild-type
male, what percentage of the F? males will have cinnabar eyes?
A) 0%
B) 25%
C) 50%
D) 75%
E) 100%

E) 100%

Normally, only female cats have the tortoiseshell phenotype because_______.
A) a male inherits only one allele of the X-linked gene
controlling hair color.
B) only males can have Barr bodies
C) the Y chromosome has a gene blocking orange coloration
D) multiple crossovers on the Y chromosome prevent orange pigment production.

A) a male inherits only one allele of the X-linked gene controlling
hair color.

In birds, sex is determined by a ZW chromosome scheme. Males are ZZ
and females are ZW. A recessive lethal allele that causes death of the
embryo is sometimes present on the Z chromosome in pigeons. What would
be the sex ratio in the offspring of a cross between a male that is
heterozygous for the lethal allele and a normal female?
A) 2:1 male to female
B) 1:2 male to female
C) 1:1 male to female
D) 4:3 male to female
E) 3:1 male to female

A) 2:1 male to female

A man who carries an allele of an X-linked gene will pass it on to ___.
A) Half of his daughters
B) All of his sons
C) All of his daughters
D) All of his children

C) All of his daughters

A man who is an achondroplastic dwarf with normal vision marries a
color-blind woman of normal height. The man's father was six feet
tall, and both the woman's parents were of average height.
Achondroplastic dwarfism is autosomal dominant, and red-green color
blindness is X-linked recessive. How many of their daughters might be
expected to be color-blind dwarfs?
A) three out of four
B) none
C) one out of four
D) half

B) none

A recessive allele on the X chromosome is responsible for red-green
color blindness in humans. A woman with normal vision whose father is
color blind marries a color-blind male. What is the probability that
this couple's first son will be color blind?
A) 1/4
B) 3/4
C) 1/2
D) 2/3

C) 1/2

In a Drosophila experiment, a cross was made between
homozygous wild-type females and yellow-bodied males. All of the
resulting F1s were phenotypically wild type. However, adult flies of
the F2 generation (resulting from matings of the F1s) had the
characteristics shown in the figure above. Consider the following questions:
(a) Is the mutant allele for yellow body recessive or dominant?
(b) Is the yellow locus autosomal (not X-linked) or X-linked?
A) (a) recessive; (b) X-linked
B) (a) recessive; (b) not X-linked
C) (a) dominant; (b) X-linked
D) (a) dominant; (b) not X-linked

A) (a) recessive; (b) X-linked

What is the definition of one map unit?
A) the recombination frequency between two genes assorting independently.
B) 1 nanometer of distance between two genes
C) a 1% frequency of recombination between two genes.
D) the physical distance between two linked genes.

C) a 1% frequency of recombination between two genes.

The greatest distance among the three genes is between a and
c. What does this mean?
A) Gene c is between a and
B) Genes are in the order: a�b�c.
C) Gene a is not recombining with
D) Gene a is between b and

B) Genes are in the order: a�b�c.

If cell X enters meiosis, and nondisjunction of one chromosome occurs
in one of its daughter cells during meiosis II, what will be the
result at the completion of meiosis?
A) All the gametes descended from cell X will be diploid.
B) Half of the gametes descended from cell X will be n + 1, and
half will be n - 1.
C) 1/4 of the gametes descended from cell X will be n + 1, 1/4
will be n - 1, and 1/2 will be n.
D) Two of the four gametes descended from cell X will be haploid,
and two will be diploid.

C) 1/4 of the gametes descended from cell X will be n + 1, 1/4 will
be n - 1, and 1/2 will be n.

Of the following human aneuploidies, which is the one that generally
has the most severe impact on the health of the individual?
A) 47, trisomy 21
B) 47, XXY
C) 47, XXX
D) 47, XYY
E) 45, X

A) 47, trisomy

A couple has a child with Down syndrome. The mother is 39 years old
at the time of delivery. Which of the following is the most probable
cause of the child's condition?
A) The woman inherited this tendency from her parents.
B) The mother had a chromosomal duplication.
C) One member of the couple underwent nondisjunction in somatic
cell production.
D) The mother most likely underwent nondisjunction during gamete production.

D) The mother most likely underwent nondisjunction during gamete production.

Correns described that the inheritance of variegated color on the
leaves of certain plants was determined by the maternal parent only.
What phenomenon does this describe?
A) mitochondrial inheritance
B) chloroplast inheritance
C) genomic imprinting
D) infectious inheritance
E) sex-linkage

B) chloroplast inheritance

Mitochondrial DNA is primarily involved in coding for proteins needed
for protein complexes of the electron transport chain and ATP
synthase. Therefore, mutations in mitochondrial genes would most affect_____.
A) DNA synthesis in cells of the immune system
B) the movement of oxygen into erythrocytes
C) the storage of urine in the urinary bladder
D) the generation of ATP in muscle cells

C) the storage of urine in the urinary bladder

The pedigree in the figure above shows the transmission of a trait in
a particular family. Based on this pattern of transmission, the trait
is most likely _____.
A) mitochondrial
B) sex-linked dominant
C) sex-linked recessive
D) autosomal dominant

A) mitochondrial

During meiosis, a defect occurs in a cell that results in the failure
of microtubules, spindle fibers, to bind at the kinetochores, a
protein structure on chromatids where the spindle fibers attach during
cell division to pull sister chromatids apart. Which of the following
is the most likely result of such a defect?
A) New microtubules with more effective binding capabilities to
kinetochores will be synthesized to compensate for the defect.
B) Excessive cell divisions will occur resulting in cancerous
tumors and an increase in the chromosome numbers known as polyploidy.
C) The defect will be bypassed in order to and ensure normal
chromosome distribution in the new cells.
D) The resulting cells will not receive the correct number of
chromosomes in the gametes, a condition known as aneuploidy.

D) The resulting cells will not receive the correct number of
chromosomes in the gametes, a condition known as aneuploidy.

Inheritance patterns cannot always be explained by Mendel�s models of
inheritance. If a pair of homologous chromosomes fails to separate
during meiosis I, select the choice
that shows the chromosome number of the four resulting gametes
with respect to the normal haploid number (n)?
A) n+1; n+1; n-1; n-1
B) n+1; n-1; n; n
C) n+1; n-1; n-1; n-1
D) n+1; n+1; n; n

A) n+1; n+1; n-1; n-1

In his transformation experiments, what did Griffith observe?
A) Mutant mice were resistant to bacterial infections.
B) Mixing a heat-killed pathogenic strain of bacteria with a
living nonpathogenic strain can convert some of the living cells into
the pathogenic form.
C) Mixing a heat-killed nonpathogenic strain of bacteria with a
living pathogenic strain makes the pathogenic strain nonpathogenic.
D) Infecting mice with nonpathogenic strains of bacteria makes
them resistant to pathogenic strains.
E) Mice infected with a pathogenic strain of bacteria can spread
the infection to other mice.

B) Mixing a heat-killed pathogenic strain of bacteria with a living
nonpathogenic strain can convert some of the living cells into the
pathogenic form.

Which of the following investigators was/were responsible for the
following discovery? In DNA from any species, the amount of adenine
equals the amount of thymine, and the amount of guanine equals the
amount of cytosine.
A) Frederick Griffith
B) Alfred Hershey and Martha Chase
C) Oswald Avery, Maclyn McCarty, and Colin MacLeod
D) Erwin Chargaff
E) Matthew Meselson and Franklin Stahl

D) Erwin Chargaff

For a science fair project, two students decided to repeat the
Hershey and Chase experiment, with modifications. They decided to
label the nitrogen of the DNA, rather than the phosphate. They
reasoned that each nucleotide has only one phosphate and two to five
nitrogens. Thus, labeling the nitrogens would provide a stronger
signal than labeling the phosphates. Why won't this experiment work?
A) Amino acids (and thus proteins) also have nitrogen atoms;
thus, the radioactivity would not distinguish between DNA and proteins
B) Although there are more nitrogens in a nucleotide, labeled
phosphates actually have sixteen extra neutrons; therefore, they are
more radioactive.
C) There is no radioactive isotope of nitrogen
D) Radioactive nitrogen has a half-life of 100,000 years, and he
material would be too dangerous for too long

A) Amino acids (and thus proteins) also have nitrogen atoms; thus,
the radioactivity would not distinguish between DNA and proteins

In the polymerization of DNA, a phosphodiester bond is formed between
a phosphate group of the nucleotide being added and _____ of the last
nucleotide in the polymer.
A) the 5' phosphate
B) C6
C) the 3' OH
D) a nitrogen from the nitrogen-containing base

C) the 3' OH

At a specific area of a chromosome, the sequence of nucleotides below
is present where the chain opens to form a replication fork: 3'
C C T A G G C T G C A A T C C 5' An RNA primer is formed
starting at the underlined T (T) of the template. Which of the
following represents the primer sequence?
A) 5' G C C T A G G 3'
B) 3' G C C T A G G 5'
C) 5' A C G T T A G G 3'
D) 5' A C G U U A G G 3'
E) 5' G C C U A G G 3'

D) 5' A C G U U A G G 3'

The difference between ATP and the nucleoside triphosphates used
during DNA synthesis is that
A) the nucleoside triphosphates have the sugar deoxyribose; ATP
has the sugar ribose.
B) the nucleoside triphosphates have two phosphate groups; ATP
has three phosphate groups.
C) ATP contains three high-energy bonds; the nucleoside
triphosphate have two.
D) ATP is found only in human cells; the nucleoside triphosphates
are found in all animal and plant cells.
E) triphosphate monomers are active in the nucleoside
triphosphates, but not in ATP.

A) the nucleoside triphosphates have the sugar deoxyribose; ATP has
the sugar ribose.

Which of the following help(s) to hold the DNA strands apart while
they are being replicated?
A) primase
B) ligase
C) DNA polymerase
D) single-strand binding proteins
E) exonuclease

D) single-strand binding proteins

In the late 1950s, Meselson and Stahl grew bacteria in a medium
containing "heavy" nitrogen (�?N) and then transferred them
to a medium containing �?N. Which of the results in the figure above
would be expected after one round of DNA replication in the presence
of �?N?
A) A
B) B
C) C
D) D
E) E

D) D

A space probe returns with a culture of a microorganism found on a
distant planet. Analysis shows that it is a carbon-based life-form
that has DNA. You grow the cells in �?N medium for several generations
and then transfer them to �?N medium. Which pattern in the figure
above would you expect if the DNA was replicated in a conservative manner?
A) A
B) B
C) C
D) D
E) E

B) B

After the first replication was observed in their experiments testing
the nature of DNA replication, Meselson and Stahl could be confident
of which of the following conclusions?
A) Replication is semi-conservative.
B) Replication is not dispersive.
C) Replication is not semi-conservative.
D) Replication is not conservative.
E) Replication is neither dispersive nor conservative.

D) Replication is not conservative.

Semiconservative replication involves a template. What is the template?
A) one strand of the DNA molecule
B) an RNA molecule
C) single-stranded binding proteins
D) DNA polymerase

A) one strand of the DNA molecule

What is the difference between the leading strand and the lagging
strand in DNA replication?
A) There are different DNA polymerases involved in the elongation
of the leading strand and the lagging strand.
B) The leading strand is synthesized continuously in the 5' ->
3' direction, while the lagging strand is synthesized discontinuously
in the 5' -> 3' direction.
C) The leading strand requires an RNA primer, whereas the lagging
strand does not.
D) The leading strand is synthesized in the 3' -> 5' direction
in a discontinuous fashion, while the lagging strand is synthesized
in the 5' -> 3' direction in a continuous fashion.

B) The leading strand is synthesized continuously in the 5' -> 3'
direction, while the lagging strand is synthesized discontinuously in
the 5' -> 3' direction.

What is a major difference between eukaryotic DNA replication and
prokaryotic DNA replication?
A) DNA polymerases of prokaryotes can add nucleotides to both 3'
and 5' ends of DNA strands, while those of eukaryotes function only
in the 5' -> 3' direction
B) Prokaryotic replication does not require a primer
C) DNA replication in prokaryotic cells is conservative. DNA
replication is eukaryotic cells is semi-conservative.
D) Prokaryotic chromosomes have a single origin of replication,
while eukaryotic chromosomes have multiple origins of replication.

D) Prokaryotic chromosomes have a single origin of replication, while
eukaryotic chromosomes have multiple origins of replication.

Telomere shortening puts a limit on the number of times a cell can
divide. Research has shown that telomerase can extend the life span of
cultured human cells. How might adding telomerase affect cellular aging?
A) Telomerase will speed up the rate of cell proliferation.
B) Telomerase eliminates telomere shortening and retards aging.
C) Telomerase shortens telomeres, which delays cellular aging.
D) Telomerase would have no effect on cellular aging.

B) Telomerase eliminates telomere shortening and retards aging.

Which of the following statements describes the eukaryotic chromosome?
A) It is composed of DNA alone.
B) The nucleosome is its most basic functional subunit.
C) The number of genes on each chromosome is different in
different cell types of an organism.
D) a single linear molecule of double-stranded DNA plus proteins.
E) Active transcription occurs on heterochromatin but not euchromatin.

D) a single linear molecule of double-stranded DNA plus proteins.

If a cell were unable to produce histone proteins, which of the
following would be a likely effect? A) There would be an
increase in the amount of "satellite" DNA produced during centrifugation.
B) The cell's DNA couldn't be packed into its nucleus.
C) Spindle fibers would not form during prophase.
D) Amplification of other genes would compensate for the lack of histones.
E) Pseudogenes would be transcribed to compensate for the
decreased protein in the cell.

B) The cell's DNA couldn't be packed into its nucleus.

Which of the following represents the order of increasingly higher
levels of organization of chromatin?
A) nucleosome, 30-nm chromatin fiber, looped domain
B) looped domain, 30-nm chromatin fiber, nucleosome
C) looped domain, nucleosome, 30-nm chromatin fiber
D) nucleosome, looped domain, 30-nm chromatin fiber
E) 30-nm chromatin fiber, nucleosome, looped domain

A) nucleosome, 30-nm chromatin fiber, looped domain

In E. coli replication the enzyme primase is used to attach a 5 to 10
base ribonucleotide strand complementary to the parental DNA strand.
The RNA strand serves as a starting point for the DNA polymerase that
replicates the DNA. If a mutation occurred in the primase gene, which
of the following would you expect?
A) Replication would only occur on the leading strand.
B) Replication would only occur on the lagging strand.
C) Replication would not occur on either the leading or lagging strand.
D) Replication would not be affected as the enzyme primase in
involved with RNA synthesis.

C) Replication would not occur on either the leading or lagging strand.

Garrod hypothesized that "inborn errors of metabolism" such
as alkaptonuria occur because
A) metabolic enzymes require vitamin cofactors, and affected
individuals have significant nutritional deficiencies.
B) enzymes are made of DNA, and affected individuals lack DNA polymerase.
C) many metabolic enzymes use DNA as a cofactor, and affected
individuals have mutations that prevent their enzymes from interacting
efficiently with DNA.
D) certain metabolic reactions are carried out by ribozymes, and
affected individuals lack key splicing factors.
E) genes dictate the production of specific enzymes, and affected
individuals have genetic defects that cause them to lack certain enzymes.

E) genes dictate the production of specific enzymes, and affected
individuals have genetic defects that cause them to lack certain enzymes.

The genetic code is essentially the same for all organisms. From
this, one can logically assume which of the following?
A) A gene from an organism can theoretically be expressed by any
other organism.
B) All organisms have experienced convergent evolution.
C) DNA was the first genetic material.
D) The same codons in different organisms translate into the
different amino acids.
E) Different organisms have different numbers of different types
of amino acids.

A) A gene from an organism can theoretically be expressed by any
other organism.

A possible sequence of nucleotides in the template strand of DNA that
would code for the polypeptide sequence phe-leu-ile-val would be
A) 5' TTG-CTA-CAG-TAG 3'.
B) 3' AAC-GAC-GUC-AUA 5'.
C) 5' AUG-CTG-CAG-TAT 3'.
D) 3' AAA-AAT-ATA-ACA 5'.
E) 3' AAA-GAA-TAA-CAA 5'.

D) 3' AAA-AAT-ATA-ACA 5'.

What amino acid sequence will be generated, based on the following
mRNA codon sequence? 5' AUG-UCU-UCG-UUA-UCC-UUG 3'
A) met-arg-glu-arg-glu-arg
B) met-glu-arg-arg-glu-leu
C) met-ser-leu-ser-leu-ser
D) met-ser-ser-leu-ser-leu
E) met-leu-phe-arg-glu-glu

D) met-ser-ser-leu-ser-leu

According to the central dogma, what molecule should go in the blank?
DNA -> ____ -> Proteins
A) tRNA
B) mtDNA
C) rRNA
D) mRNA

D) mRNA

Which of the following statements best describes the termination of
transcription in prokaryotes?
A) RNA polymerase transcribes through the polyadenylation signal,
causing proteins to associate with the transcript and cut it free from
the polymerase.
B) RNA polymerase transcribes through the terminator sequence,
causing the polymerase to separate from the DNA and release the transcript.
C) RNA polymerase transcribes through an intron, and the snRNPs
cause the polymerase to let go of the transcript.
D) Once transcription has initiated, RNA polymerase transcribes
until it reaches the end of the chromosome.
E) RNA polymerase transcribes through a stop codon, causing the
polymerase to stop advancing through the gene and release the mRNA.

B) RNA polymerase transcribes through the terminator sequence,
causing the polymerase to separate from the DNA and release the transcript.

Alternative RNA splicing ________.
A) can allow the production of similar proteins from different RNAs
B) can allow the production of proteins of different sizes and
functions from a single mRNA
C) is a mechanism for increasing the rate of translation
D) increases the rate of transcription

B) can allow the production of proteins of different sizes and
functions from a single mRNA

Which of the following statements best describes the termination of
transcription in prokaryotes?
A) RNA polymerase transcribes through the polyadenylation signal,
causing proteins to associate with the transcript and cut it free from
the polymerase.
B) RNA polymerase transcribes through the terminator sequence,
causing the polymerase to separate from the DNA and release the transcript.
C) RNA polymerase transcribes through an intron, and the snRNPs
cause the polymerase to let go of the transcript.
D) Once transcription has initiated, RNA polymerase transcribes
until it reaches the end of the chromosome.
E) RNA polymerase transcribes through a stop codon, causing the
polymerase to stop advancing through the gene and release the mRNA.

B) RNA polymerase transcribes through the terminator sequence,
causing the polymerase to separate from the DNA and release the transcript.

In an experimental situation, a student researcher inserts an mRNA
molecule into a eukaryotic cell after he has removed its 5' cap and
poly-A tail. Which of the following would you expect him to find?
A) The mRNA could not exit the nucleus to be translated.
B) The cell recognizes the absence of the tail and polyadenylates
the mRNA.
C) The molecule is digested by restriction enzymes in the nucleus.
D) The molecule is digested by exonucleases since it is no longer
protected at the 5' end.
E) The molecule attaches to a ribosome and is translated, but
more slowly.

D) The molecule is digested by exonucleases since it is no longer
protected at the 5' end.

5' UTR E? I? E? I? E? I? E? UTR 3'
Which components of the previous molecule will also be found in
mRNA in the cytosol?
A) 5' UTR I? I? I? UTR 3'
B) 5' E? E? E? E? 3'
C) 5' UTR E? E? E? E? UTR 3'
D) 5' I? I? I? 3'
E) 5' E? I? E? I? E? I? E? 3'

C) 5' UTR E? E? E? E? UTR 3'

A particular triplet of bases in the coding sequence of DNA is AAA.
The anticodon on the tRNA that binds the mRNA codon is
A) TTT
B) UUA
C) UUU
D) AAA
E) either UAA or TAA, depending on first base wobble

D) AAA

Accuracy in the translation of mRNA into the primary structure of a
polypeptide depends on specificity in the
A) binding of ribosomes to mRNA.
B) shape of the A and P sites of ribosomes.
C) bonding of the anticodon to the codon.
D) attachment of amino acids to tRNAs.
E) bonding of the anticodon to the codon and the attachment of
amino acids to tRNAs.

E) bonding of the anticodon to the codon and the attachment of amino
acids to tRNAs.

Which one of the following statements about RNA processing is true?
A) Exons are cut out before mRNA leaves the nucleus.
B) Ribozymes may function in RNA splicing.
C) RNA splicing can be catalyzed by tRNA.
D) A primary transcript is often much shorter than the final RNA
molecule that leaves the nucleus.

B) Ribozymes may function in RNA splicing.

A primary transcript in the nucleus of a eukaryotic cell is _____ the
functional mRNA, while a primary transcript in a prokaryotic cell is
_____ the functional mRNA.
A) the same size as; smaller than
B) larger than; the same size as
C) larger than; smaller than
D) the same size as; larger than

B) larger than; the same size as

In the structural organization of many eukaryotic genes, individual
exons may be related to which of the following?
A) the sequence of the intron that immediately precedes each exon
B) the number of polypeptides making up the functional protein
C) the various domains of the polypeptide product
D) the number of start sites for transcription

C) the various domains of the polypeptide product

In eukaryotes there are several different types of RNA polymerase.
Which type is involved in transcription of mRNA for a globin protein?
A) ligase
B) RNA polymerase I
C) RNA polymerase II
D) RNA polymerase III
E) primase

C) RNA polymerase II

Transcription in eukaryotes requires which of the following in
addition to RNA polymerase?
A) the protein product of the promoter
B) start and stop codons
C) ribosomes and tRNA
D) several transcription factors (TFs)
E) aminoacyl synthetase

D) several transcription factors (TFs)

Which of the following best describes the significance of the TATA
box in eukaryotic promoters?
A) It sets the reading from of the mRNA
B) It is the recognition site for ribosomal binding
C) Its significance has not yet been determined
D) It is the recognition site for a specific transcription factor

D) It is the recognition site for a specific transcription factor

Which of the following does not occur in prokaryotic gene expression,
but does occur in eukaryotic gene expression?
A) mRNA, tRNA, and rRNA are transcribed
B) RNA polymerase binds to the promoter
C) RNA polymerase requires a primer to elongate the molecule
D) A cap is added to the 5' end of the mRNA

D) A cap is added to the 5' end of the mRNA

A ribozyme is _____.
A) a catalyst that uses RNA as a substrate
B) an enzyme that catalyzes the association between the large and
small ribosomal subunits
C) an RNA with catalytic activity
D) an enzyme that synthesizes RNA as a part of the transcription process

C) an RNA with catalytic activity

Codons are three-base sequences that specify the addition of a single
amino acid. How do eukaryotic codons and prokaryotic codons compare?
A) Prokaryotic codons usually contain different bases than those
of eukaryotes.
B) Prokaryotic codons usually specify different amino acids than
those of eukaryotes.
C) The translation of codons is mediated by tRNAs in eukaryotes,
but translation requires no intermediate molecules such as tRNAs in prokaryotes.
D) Codons are a nearly universal language among all organisms.

D) Codons are a nearly universal language among all organisms.

Which of the following occurs in prokaryotes but not in eukaryotes?
A) translation in the absence of a ribosome
B) post-transcriptional splicing
C) concurrent transcription and translation
D) gene regulation

C) concurrent transcription and translation

Refer to the figure above. What would the anticodon be for a tRNA
that transports phenylalanine to a ribosome?
A) UUU
B) AAA
C) TTT
D) CCC

B) AAA

Which of the following contradicts the one-gene, one-enzyme hypothesis?
A) A mutation in a single gene can result in a defective protein.
B) Alkaptonuria results when individuals lack a single enzyme
involved in the catalysis of homogentisic acid.
C) Sickle-cell anemia results in defective hemoglobin.
D) A single antibody gene can code for different related
proteins, depending on the splicing that takes place post-transcriptionally.

D) A single antibody gene can code for different related proteins,
depending on the splicing that takes place post-transcriptionally.

Which of the following is directly related to a single amino acid?
A) the base sequence of the tRNA
B) the amino acetyl tRNA synthase
C) the three-base sequence of mRNA
D) the complementarity of DNA and RNA

C) the three-base sequence of mRNA

In the process of transcription, _____.
A) DNA is replicated
B) RNA is synthesized
C) proteins are synthesized
D) mRNA attaches to ribosomes

B) RNA is synthesized

Codons are part of the molecular structure of _____.
A) a protein
B) mRNA
C) tRNA
D) rRNA

B) mRNA

What does it mean when we say the genetic code is redundant?
A) A single codon can specify the addition of more than one amino acid.
B) The genetic code is different for different domains of organisms.
C) The genetic code is universal (the same for all organisms).
D) More than one codon can specify the addition of the same amino acid.

D) More than one codon can specify the addition of the same amino acid.

Once researchers identified DNA as the unit of inheritance, they
asked how information was transferred from the DNA in the nucleus to
the site of protein synthesis in the cytoplasm. What is the mechanism
of information transfer in eukarotes?
A) DNA from a single gene is replicated and transferred to the
cytoplasm, where it serves as a template for protein synthesis.
B) Messenger RNA is transcribed from a single gene and transfers
information from the DNA in the nucleus to the cytoplasm, where
protein synthesis takes place.
C) Proteins transfer information from the nucleus to the
ribosome, where protein synthesis takes place.
D) Transfer RNA takes information from DNA directly to a
ribosome, where protein synthesis takes place.

B) Messenger RNA is transcribed from a single gene and transfers
information from the DNA in the nucleus to the cytoplasm, where
protein synthesis takes place.

The figure above shows a simple metabolic pathway. According to
Beadle and Tatum's hypothesis, how many genes are necessary for this pathway?
A) 1
B) 2
C) 3
D) It cannot be determined from the pathway.

B) 2

Refer to the metabolic pathway illustrated above. If A, B, and C are
all required for growth, a strain that is mutant for the gene-encoding
enzyme A would be able to grow on medium supplemented with _____.
A) nutrient A only
B) nutrient B only
C) nutrient C only
D) nutrients A and C

B) nutrient B only

Refer to the metabolic pathway illustrated above. If A, B, and C are
all required for growth, a strain mutant for the gene-encoding enzyme
B would be able to grow on medium supplemented with _____.
A) nutrient A only
B) nutrient B only
C) nutrient C only
D) nutrients A and C

C) nutrient C only

A particular triplet of bases in the template strand of DNA is 5' AGT
3'. The corresponding codon for the mRNA transcribed is
A) 3' UCA 5'.
B) 3' UGA 5'.
C) 5' TCA 3'.
D) 3' ACU 5'.

A) 3' UCA 5'.

Which of the following statements describes chromatin?
A) Heterochromatin is composed of DNA, whereas euchromatin is
made of DNA and RNA.
B) Both heterochromatin and euchromatin are found in the cytoplasm.
C) Heterochromatin is highly condensed, whereas euchromatin is
less compact.
D) Euchromatin is not transcribed, whereas heterochromatin is transcribed.
E) Only euchromatin is visible under the light microscope.

C) Heterochromatin is highly condensed, whereas euchromatin is less compact.

Telomere shortening is a problem in which types of cells?
A) only prokaryotic cells
B) only eukaryotic cells
C) cells in prokaryotes and eukaryotes

B) only eukaryotic cells

Where of the following cells have reduced or very little active
telomerase activity?
A) most normal germ cells
B) most cancer cells
C) most normal somatic cells

C) most normal somatic cells

What is a telomere?
A) the mechanism that holds two sister chromatids together
B) DNA replication during telophase
C) the site of origin of DNA replication
D) the ends of linear chromosomes

D) the ends of linear chromosomes

DNA is synthesized through a process known as _____.
A) semiconservative replication
B) conservative replication
C) translation
D) transcription

A) semiconservative replication

DNA contains the template needed to copy itself, but it has no
catalytic activity in cells. What enzyme catalyzesthe formation
of phosphodiester bonds between adjacent nucleotides in the DNA
polymer being formed?
A) ribozymes
B) DNA polymerase
C) deoxyribonucleotide triphosphates
D) ATP

B) DNA polymerase

What provides the energy for the polymerization reactions in DNA synthesis?
A) DNA polymerase
B) the deoxyribonucleotide triphosphate substrates
C) breaking down the hydrogen bonds between complementary DNA strands
D) ATP

B) the deoxyribonucleotide triphosphate substrates

Refer to the figure above. What bases will be added to the primer as
DNA replication proceeds? The bases should appear in the new strand in
the order that they will be added starting at the 3' end of the primer.
A) C, A, G, C, A, G, A
B) T, C, T, G, C, T, G
C) A, G, A, C, G, A, C
D) G, T, C, G, T, C, T

C) A, G, A, C, G, A, C

You briefly expose bacteria undergoing DNA replication to
radioactively labeled nucleotides. When you centrifuge the DNA
isolated from the bacteria, the DNA separates into two classes. One
class of labeled DNA includes very large molecules (thousands or even
millions of nucleotides long), and the other includes short stretches
of DNA (several hundred to a few thousand nucleotides in length).
These two classes of DNA probably represent
A) leading strands and Okazaki fragments.
B) lagging strands and Okazaki fragments.
C) Okazaki fragments and RNA primers.
D) leading strands and RNA primers.
E) RNA primers and mitochondrial DNA.

A) leading strands and Okazaki fragments.

Within a double-stranded DNA molecule, adenine forms hydrogen bonds
with thymine and cytosine forms hydrogen bonds with guanine. This
arrangement _______.
A) determines the type of protein produced
B) determines the tertiary structure of a DNA molecule
C) permits complementary base pairing
D) allows variable width of the double helix

C) permits complementary base pairing

The leading and the lagging strands differ in that _____.
A) the leading strand is synthesized in the same direction as the
movement of the replication fork, and the lagging strand is
synthesized in the opposite direction
B) the leading strand is synthesized by adding nucleotides to the
end of the growing strand, and the lagging strand is synthesized by
adding nucleotides to the end
C) the lagging strand is synthesized continuously, whereas the
leading strand is synthesized in short fragments that are ultimately
stitched together
D) the leading strand is synthesized at twice the rate of the
lagging strand

A) the leading strand is synthesized in the same direction as the
movement of the replication fork, and the lagging strand is
synthesized in the opposite direction

A new DNA strand elongates only in the 5' to 3' direction because _____.
A) DNA polymerase begins adding nucleotides at the 5' end of the template
B) the polarity of the DNA molecule prevents addition of
nucleotides at the 3' end
C) replication must progress toward the replication fork
D) DNA polymerase can add nucleotides only to the free 3' end

D) DNA polymerase can add nucleotides only to the free 3' end

What is the function of topoisomerase?
A) relieving strain in the DNA ahead of the replication fork
B) elongating new DNA at a replication fork by adding nucleotides
to the existing chain
C) unwinding of the double helix
D) stabilizing single-stranded DNA at the replication fork

A) relieving strain in the DNA ahead of the replication fork

What is the role of DNA ligase in the elongation of the lagging
strand during DNA replication?
A) It synthesizes RNA nucleotides to make a primer.
B) It joins Okazaki fragments together.
C) It unwinds the parental double helix.
D) It stabilizes the unwound parental DNA.

B) It joins Okazaki fragments together.

In E. coli, to repair a thymine dimer by nucleotide excision
repair, in which order do the necessary enzymes act?
A) nuclease, DNA polymerase III, RNA primase
B) helicase, DNA polymerase I, DNA ligase
C) DNA ligase, nuclease, helicase
D) nuclease, DNA polymerase I, DNA ligase

D) nuclease, DNA polymerase I, DNA ligase

Replication in prokaryotes differs from replication in eukaryotes for
which of the following reasons?
A) Prokaryotic chromosomes have histones, whereas eukaryotic
chromosomes do not.
B) Prokaryotic chromosomes have a single origin of replication,
whereas eukaryotic chromosomes have many.
C) The rate of elongation during DNA replication is slower in
prokaryotes than in eukaryotes.
D) Prokaryotes produce Okazaki fragments during DNA replication,
but eukaryotes do not.

B) Prokaryotic chromosomes have a single origin of replication,
whereas eukaryotic chromosomes have many.

What is meant by the description "antiparallel" regarding
the strands that make up DNA?
A) The twisting nature of DNA creates nonparallel strands.
B) The 5' to 3' direction of one strand runs counter to the to
direction of the other strand.
C) Base pairings create unequal spacing between the two DNA strands.
D) One strand contains only purines and the other contains only pyrimidines.

B) The 5' to 3' direction of one strand runs counter to the to
direction of the other strand.

Suppose you are provided with an actively dividing culture of E.coli
bacteria to which radioactive thymine has been added. What would
happen if a cell replicates once in the presence of this radioactive base?
A) Neither of the two daughter cells would be radioactive
B) One of the daughter cells, but not the other, would have
radioactive DNA.
C) All four bases of the DNA would be radioactive.
D) DNA in both daughter cells would be radioactive.

D) DNA in both daughter cells would be radioactive.

In E.coli, there is a mutation in a gene called dnaB that alters the
helicase that normally acts at the origin. Which of the following
would you expect as a result of the this mutation?
A) No replication fork will be formed.
B) Additional proofreading will occur.
C) R�plication will occur via RNA polymerase alone.
D) Replication will require a DNA template from another source.

A) No replication fork will be formed.

In E.coli, which enzyme catalyzes the elongation of a new DNA strand
in the 5' --> 3' direction?
A) helicase
B) primase
C) DNA ligase
D) DNA polymerase III

D) DNA polymerase III

Eukaryotic telomeres replicate differently than the rest of the
chromosome. This is a consequence of which of the following?
A) DNA polymerase that cannot replicate the leading strand
template to its 5' end
B) gaps left at the 5' end of the lagging strand
C) gaps left at the 3' end of the lagging strand because of the
need for a primer
D) the evolution of telomerase enzyme

B) gaps left at the 5' end of the lagging strand

How does the enzyme telomerase meet the challenge of replicating the
ends of linear chromosomes?
A) It adds numerous GC pairs, which resist hydrolysis and maintain
chromosome integrity.
B) It adds a single 5' cap structure that resists degradation by nucleases.
C) It catalyzes the lengthening of telomeres, compensating for the
shortening that could occur during replication without telomerase activity.
D) It causes specific double-strand DNA breaks that result in
blunt ends on both stands.

C) It catalyzes the lengthening of telomeres, compensating for the
shortening that could occur during replication without telomerase activity.

Replication in prokaryotes differs from replication in eukaryotes for
which of the following reasons?
A) The rate of elongation during DNA replication is slower in
prokaryotes than in eukaryotes.
B) Prokaryotes produce Okazaki fragments during DNA replication,
but eukaryotes do not.
C) Prokaryotic chromosomes have a single origin of replication,
whereas eukaryotic chromosomes have many.
D) Prokaryotic chromosomes have histones, whereas eukaryotic
chromosomes do not.

C) Prokaryotic chromosomes have a single origin of replication,
whereas eukaryotic chromosomes have many.

Hersey and Chase set out to determine what molecule served as the
unit of inheritance. They completed a series of experiments in which
E.coli was infected by a T2 virus. Which molecular component of the T2
virus actually ended up inside the cell?
A) ribosome
B) DNA
C) RNA
D) protein

B) DNA

Cytosine makes up 42% of the nucleotides in a sample of DNA from an
organism. Approximately what percentage of the nucleotides in this
sample will be thymine?
A) 16%
B) 58%
C) 42%
D) 8%

D) 8%

It became apparent to Watson and Crick after completion of their
model that the DNA molecule could carry a vast amount of hereditary
information in which of the following?
A) sequence of bases
B) phosphate-sugar backbones
C) complementary pairing of bases
D) side groups of nitrogenous bases
E) different five-carbon sugars

A) sequence of bases

In an analysis of the nucleotide composition of DNA, which of the
following will be found?
A) A = C
B) A = G and C = T
C) A + C = G + T
D) G + C = T + A

C) A + C = G + T

How do we describe transformation in bacteria?
A) the creation of a strand of DNA from an RNA molecule
B) the creation of a strand of RNA from a DNA molecule
C) the infection of cells by a phage DNA molecule
D) the type of semiconservative replication shown by DNA
E) assimilation of external DNA into a cell

E) assimilation of external DNA into a cell

After mixing a heat-killed, phosphorescent strain of bacteria with a
living nonphosphorescent strain, you discover that some of the living
cells are now phosphorescent. Which observations would provide the
best evidence that the ability to fluoresce is a heritable trait?
A) DNA passed from the heat-killed strain to the living strain.
B) Protein passed from the heat-killed strain to the living strain.
C) The phosphorescence in the living strain is especially bright.
D) Phosphorescence in descendants of the living cells.

D) Phosphorescence in descendants of the living cells.

In trying to determine whether DNA or protein is the genetic
material, Hershey and Chase made use of which of the following facts?
A) DNA contains sulfur, whereas protein does not.
B) DNA contains phosphorus, whereas protein does not.
C) DNA contains nitrogen, whereas protein does not.
D) DNA contains purines, whereas protein includes pyrimidines.

B) DNA contains phosphorus, whereas protein does not.

What is a syndrome?
A) a characteristic facial appearance
B) a group of traits, all of which must be present if an
aneuploidy is to be diagnosed
C) a group of traits typically found in conjunction with a
particular chromosomal aberration or gene mutation
D) a characteristic trait usually given the discoverer's name
E) a characteristic that only appears in conjunction with one
specific aneuploidy

C) a group of traits typically found in conjunction with a particular
chromosomal aberration or gene mutation

One possible result of chromosomal breakage is for a fragment to join
a nonhomologous chromosome. What is this alteration called?
A) deletion
B) transversion
C) inversion
D) translocation
E) duplication

D) translocation

What is the reason that closely linked genes are typically inherited together?
A) They are located close together on the same chromosome.
B) The number of genes in a cell is greater than the number of chromosomes.
C) Chromosomes are unbreakable.
D) Alleles are paired together during meiosis.
E) Genes align that way during metaphase I of meiosis.

A) They are located close together on the same chromosome.

In Drosophila melanogaster, vestigial wings are caused by a recessive
allele of a gene that is linked to a gene with a recessive allele that
causes black body color. Morgan crossed black-bodied, normal-winged
females and gray-bodied, vestigial-winged males. The F1 were all gray
bodied, normal winged. The F1 females were crossed to homozygous
recessive males to produce test cross progeny. Morgan calculated the
map distance to be 17 map units. Which of the following is correct
about the test cross progeny?
A) black-bodied, normal-winged flies = 17% of the total
B) gray-bodied, normal-winged flies PLUS black-bodied,
vestigial-winged flies = 17% of the total
C) black-bodied, vestigial-winged flies = 17% of the total
D) black-bodied, normal-winged flies PLUS gray-bodied,
vestigial-winged flies = 17% of the total

B) gray-bodied, normal-winged flies PLUS black-bodied,
vestigial-winged flies = 17% of the total

Recombination between linked genes comes about for what reason?
A) Mutation on one homolog is different from that on the other homolog.
B) Independent assortment sometimes fails because Mendel had not
calculated appropriately.
C) When genes are linked they always "travel" together
at anaphase.
D) Crossovers between these genes result in chromosomal exchange.
E) Nonrecombinant chromosomes break and then re-join with one another.

D) Crossovers between these genes result in chromosomal exchange.

Map units on a linkage map cannot be relied upon to calculate
physical distances on a chromosome for which of the following reasons?
A) The frequency of crossing over varies along the length of the chromosome.
B) The relationship between recombination frequency and map units
is different in every individual.
C) Physical distances between genes change during the course of
the cell cycle.
D) The gene order on the chromosomes is slightly different in
every individual.
E) Linkage map distances are identical between males and females.

A) The frequency of crossing over varies along the length of the chromosome.

E) A and G

Sturtevant provided genetic evidence for the existence of four pairs
of chromosomes in Drosophila in which of these ways? A)
There are four major functional classes of genes in Drosophila.
B) Drosophila genes cluster into four distinct groups of linked genes.
C) The overall number of genes in Drosophila is a multiple of four.
D) The entire Drosophila genome has approximately 400 map units.
E) Drosophila genes have, on average, four different alleles.

B) Drosophila genes cluster into four distinct groups of linked genes.

Which of the following statements is true of linkage?
A) The closer two genes are on a chromosome, the lower the
probability that a crossover will occur between them.
B) The observed frequency of recombination of two genes that are
far apart from each other has a maximum value of 100%.
C) All of the traits that Mendel studied�seed color, pod shape,
flower color, and others�are due to genes linked on the same chromosome.
D) Linked genes are found on different chromosomes.
E) Crossing over occurs during prophase II of meiosis.

A) The closer two genes are on a chromosome, the lower the
probability that a crossover will occur between them.

How would one explain a testcross involving F? dihybrid flies in
which more parental-type offspring than recombinant-type offspring are
produced? A) The two genes are closely linked on the
same chromosome.
B) The two genes are linked but on different chromosomes.
C) Recombination did not occur in the cell during meiosis.
D) The testcross was improperly performed.
E) Both of the characters are controlled by more than one gene.

A) The two genes are closely linked on the same chromosome.

A man who carries an alley of an X-linked gene will pass it on to ___.
A) Half of his daughters
B) All of his sons
C) All of his daughters
D) All of his children

C) All of his daughters

Glucose-6-phosphate dehydrogenase deficiency (G6PD) is inherited as a
recessive allele of an X-linked gene in humans. A woman whose father
suffered from G6PD marries a normal man.
i) What proportion of their sons is expected to be G6PD?
ii) If the husband was not normal but was G6PD deficient, would
you change your answer in part i?
A) (i) 1/2 (ii) Yes
B) (i) 100% (ii) No
C) (i) 0 (ii) No
D) (i) 1/2 (ii) No

D) (i) 1/2 (ii) No

A mutant bacterial cell has a defective aminoacyl -tRNA synthetase
that attaches a lysine to tRNAs with the anticodon AAA instead of the
normal phenylalanine. The consequence of this for the cell will be that
A) none of the proteins in the cell will contain phenylalanine.
B) proteins in the cell will include lysine instead of
phenylalanine at amino acid positions specified by the codon UUU.
C) the cell will compensate for the defect by attaching
phenylalanine to tRNAs with lysine-specifying anticodons.
D) the ribosome will skip a codon every time a UUU is encountered.

B) proteins in the cell will include lysine instead of phenylalanine
at amino acid positions specified by the codon UUU.

There are 61 mRNA codons that specify an amino acid, but only 45
tRNAs. This is best explained by the fact that
A) some tRNAs have anticodons that recognize four or more
different codons.
B) the rules for base pairing between the third base of a codon
and tRNA are flexible.
C) many codons are never used, so the tRNAs that recognize them
are dispensable.
D) the DNA codes for all 61 tRNAs but some are then destroyed.
E) competitive exclusion forces some tRNAs to be destroyed by nucleases.

B) the rules for base pairing between the third base of a codon and
tRNA are flexible.

The release factor (RF) ____.
A) It separates tRNA in the A site from the growing polypeptide.
B) It binds to the stop codon in the A site in place of a tRNA.
C) It releases the amino acid from its tRNA to allow the amino
acid to form a peptide bond.
D) It supplies a source of energy for termination of translation.
E) It releases the ribosome from the ER to allow polypeptides into
the cytosol.

B) It binds to the stop codon in the A site in place of a tRNA.

The anticodon loop of the first tRNA that will complement this mRNA is
A) 3' GGC 5'
B) 5' GGC 3'
C) 5' ACG 3'
D) 5' UGC 3'
E) 3' UGC 5'

A) 3' GGC 5'

What type of bonding is responsible for maintaining the shape of the
tRNA molecule shown in the figure above?
A) covalent bonding between sulfur atoms
B) ionic bonding between phosphates
C) hydrogen bonding between base pairs
D) van der Waals interactions between hydrogen atoms
E) peptide bonding between amino acids

C) hydrogen bonding between base pairs

The figure represents tRNA that recognizes and binds a particular
amino acid (in this instance, phenylalanine). Which codon on the mRNA
strand codes for this amino acid?
A) UGG
B) GUG
C) GUA
D) UUC
E) CAU

D) UUC

The tRNA shown in the figure has its 3' end projecting beyond its 5'
end. What will occur at this 3' end?
A) The codon and anticodon complement one another.
B) The amino acid binds covalently.
C) The excess nucleotides (ACCA) will be cleaved off at the ribosome.
D) The small and large subunits of the ribosome will attach to it.
E) The 5' cap of the mRNA will become covalently bound

B) The amino acid binds covalently.

Translation requires ____.
A) mRNA, tRNA, DNA, and rRNA
B) mRNA, tRNA, and rRNA
C) mRNA, tRNa, and DNA
D) mRNA, DNA, and rRNA

B) mRNA, tRNA, and rRNA

Once a peptide has been formed between the amino acid attached to the
tRNa in the P site and the amino acid associated with the tRNAi in the
A site, what occurs next?
A) initiation
B) reading of the next codon of mRNA
C) tranlocation
D) The codon-anticodon hydrogen bonds holding the tRNA in the A
site are broken

C) tranlocation

Which one of the following, if missing, would usually prevent
translation from starting?
A) poly- A tail
B) 5' cap
C) AUG codon
D) exon

C) AUG codon

Put the following events of elongation in prokaryotic translation in
chronological order.
1. Binding of mRNA with small ribosomal subunit
2. Recognition of initiation codon
3. Complementary base pairing between initiator codon and
anticodon of initiator tRNA
4. Base pairing of the mRNA codon following the initiator codon
with its complementary tRNA
5. Attachment of the large subunit
A) 5,4,3,2,1
B) 1,2,3,5,4
C) 1,2,3,4,5
D) 2,1,4,3,5

B) 1,2,3,5,4

How does termination of translation take place?
A) The poly-A tail is reached
B) A stop codon is reached
C) The 5' cap is reached
D) The end of the mRNA molecule is reached

B) A stop codon is reached

Post-translational modifications of proteins may include the ____.
A) addition of carbohydrates to form a glycoprotein
B) addition of a poly-A tail
C) addition of a 5' cap
D) removal of introns

A) addition of carbohydrates to form a glycoprotein

During elongation, which site in the ribosome represents the location
where ac codon is being read?
A) E site
B) A site
C) P site
D) the small ribosomal subunit

B) A site

What must occur before a newly made polypeptide is secreted from a cell?
A) Its signal sequence must target it to the ER, after which it
goes to the Golgi.
B) Its signal sequence must target it to the plasma membrane,
where it causes exocytosis
C) Its signal sequence must be cleaved off before the polypeptide
can enter the ER
D) It must be translated by a ribosome that remains free within
the cytosol

A) Its signal sequence must target it to the ER, after which it goes
to the Golgi.

A part of an mRNA molecule with the following sequence is being read
by a ribosome: 5' CCG-ACG 3' (mRNA). The following charged transfer
RNA molecules (with their anticodons shown in the 3' to 5' direction)
are available. Two of them can correctly match the mRNA so that a
dipeptide can form.
The dipeptide that will form will be
A) cysteine-alanine.
B) proline-threonine.
C) glycine-cysteine.
D) alanine-alanine.
E) threonine-glycine.

B) proline-threonine.

Which of the following is the first event to take place in
translation in eukaryotes?
A) elongation of the polypeptide
B) base pairing of activated methionine-tRNA to AUG of the
messenger RNA
C) binding of the larger ribosomal subunit to smaller ribosomal subunits
D) covalent bonding between the first two amino acids
E) the small subunit of the ribosome recognizes and attaches to
the 5' cap of mRNA

E) the small subunit of the ribosome recognizes and attaches to the
5' cap of mRNA

A signal peptide ____.
A) signals the initiation of transcription
B) Helps target a protein to the ER
C) Directs an mRNA molecule into a cisternal space of the ER
D) terminates translation of messenger RNA

B) Helps target a protein to the ER

Which of the following statements is true about protein synthesis in prokaryotes?
A) Extensive RNA processing is required before prokaryotic
transcripts can be translated.
B) Translation can begin while transcription is still in progress.
C) Prokaryotic cells have complicated mechanisms for targeting
proteins to the appropriate cellular organelles.
D) Translation requires antibiotic activity.
E) Unlike eukaryotes, prokaryotes require no initiation or
elongation factors.

B) Translation can begin while transcription is still in progress.

The most commonly occurring mutation in people with cystic fibrosis
is a deletion of a single codon. This results in
A) a base-pair substitution.
B) a nucleotide mismatch.
C) a frameshift mutation.
D) a polypeptide missing an amino acid.
E) a nonsense mutation.

D) a polypeptide missing an amino acid.

How might a single base substitution in the sequence of a gene affect
the amino acid sequence of a protein encoded by the gene, and why?
A) The amino acid sequence would be substantially altered, because
the reading frame would change with a single base substitution.
B) Only a single amino acid could change, because the reading
frame is unaffected.
C) All amino acids following the substitution would be affected,
because the reading frame would be shifted.
D) It is not possible for a single base substitution to affect
protein structure, because each codon is three bases long.

B) Only a single amino acid could change, because the reading frame
is unaffected.

What do we mean when we use the terms monohybrid cross and dihybrid cross?
A) A monohybrid cross results in a 9:3:3:1 ration whereas a
dihybrid cross gives a 3:1 ratio.
B) A monohybrid cross involves a single parent, whereas a dihybrid
cross involves two parents.
C) A dihybrid cross involves organisms that are heterozygous for
two characters that are being studied, and a monohybrid cross
involves organisms that are heterozygous for only one character being studied.
D) A monohybrid cross is performed for one generation, whereas a
dihybrid cross is performed for two generations.

C) A dihybrid cross involves organisms that are heterozygous for two
characters that are being studied, and a monohybrid cross involves
organisms that are heterozygous for only one character being studied.

The fact that all seven of the pea plant traits studied by Mendel
obeyed the principle of independent assortment most probably indicates
which of the following?
A) All of the genes controlling the traits were located on the
same chromosome
B) None of the traits obeyed the law of segregation
C) All of the genes controlling the traits behaved as if they were
on different chromosomes
D) The diploid number of chromosomes in the pea plant was 7

C) All of the genes controlling the traits behaved as if they were on
different chromosomes

In a particular plant, leaf color is controlled by gene locus D.
Plants with at least one allele D have dark green leaves, and plants
with the homozygous recessive dd genotype have light green leaves. A
true-breeding dark-leaved plant is crossed with a light-leaved one,
and the F? offspring is allowed to self-pollinate. The predicted
outcome of the F? is diagrammed in the Punnett square shown in Figure
14.1, where 1, 2, 3, and 4 represent the genotypes corresponding to
each box within the square.
Which of the boxes marked 1-4 correspond to plants with dark leaves?
A) 1 only
B) 1 and 2
C) 2 and 3
D) 4 only
E) 1, 2, and 3

E) 1, 2, and 3

Albinism is an autosomal (not sex-linked) recessive trait. A man and
woman are both of normal pigmentation and have one child out of three
who is albino (without melanin pigmentation). What are the genotypes
of the albino's parents?
A) Both are heterozygous
B) One parent must be homozygous for the recessive allele; the
other parent must be homozygous dominant, homozygous recessive, or heterozygous.
C) One parent must be homozygous dominant; the other parent must
be heterozygous.
D) One parent must be heterozygous; the other parent can be
homozygous dominant, homozygous recessive, or heterozygous.

A) Both are heterozygous

Black fur in mice (B) is dominant to brown fur (b). Short tails (T)
are dominant to long tails (t). What fraction of the progeny of
crosses BbTt x BBtt will be expected to have black fur and long tails?
A) 9/16
B) 1/2
C) 3/8
D) 1/16

B) 1/2

A woman who has blood type A positive has a daughter who is type O
positive and a son who is type B negative. Rh positive is a trait that
shows simple dominance over Rh negative. Which of the following is a
possible phenotype for the father?
A) A negative
B) O negative
C) B positive
D) AB negative
E) impossible to determine

C) B positive

In humans, ABO blood types refer to glycoproteins in the membranes of
red blood cells. There are three allele for this autosomal gene: IA,
IB, and i. The IA allele codes for the A glycoprotein. The IB allele
codes for the B glycoprotein, and the i allele doesn't code for any
membrane glycoprotein. IA and IB are codominant, and i is recessive to
both IA and IB. People with type A blood have the genotypes IAIA or
IAi, people with type B blood are IBIB or IBi, people with type AB
blood are IAIB, and people with type O blood are ii. If a woman with
type AB blood marries a man with type O blood, which of the following
blood types could their children possibly have?
A) AB and O
B) A and B
C) A, B, AB, and O
D) A, B, and O

B) A and B

The following question refer to the pedigree chart in Figure 14.2 for
a family, some of whose members exhibit the dominant trait, W.
Affected individuals are indicated by a dark square or circle.
What is the genotype of individual II-5?
A) WW
B) Ww
C) ww
D) WW or ww
E) ww or Ww

C) ww

What is the likelihood that the progeny of IV-3 and IV-4 will have
the trait?
A) 0%
B) 25%
C) 50%
D) 75%
E) 100%

C) 50%

What is the probability that individual III-1 is Ww?
A) 3/4
B) 1/4
C) 2/4
D) 2/3
E) 1

E) 1

Use the following pedigree (Figure 14.3) for a family in which
dark-shaded symbols represent individuals with one of the two major
types of colon cancer. Numbers under the symbols are the individual's
age at the time of diagnosis.
From this pedigree, how does this trait seem to be inherited?
A) from mothers
B) as an autosomal recessive
C) as a result of epistasis
D) as an autosomal dominant
E) as an incomplete dominant

D) as an autosomal dominant

Which of the following statements is a correct explanation for the
observation that all offspring exhibit a phenotype for a particular
trait that appears to be a blend of the two parental varieties?
A) Neither of the parental genes is dominant over the other
B) The genes are linked and do not separate during meiosis
C) The genes for the trait are recessive in both of the parents
D) The genes for the trait are dominant in both parents

A) Neither of the parental genes is dominant over the other

A gene for the MN blood group has codominant alleles M and N. If both
children are of blood type M, which of the following is possible?
A) Each parent is either M or MN.
B) Each parent must be type M
C) Neither parent can have the N allele
D) Both children are heterozygous for this gene

A) Each parent is either M or MN.

In certain plants, tall is dominant to short. If a heterozygous plant
is crossed with a homozygous tall plant, what is the probability that
the offspring will be short?
A) 1
B) 1/2
C) 1/4
D) 1/6
E) 0

E) 0

In the cross AaBbCc � AaBbCc, what is the probability of producing
the genotype AABBCC?
A) 1/4
B) 1/8
C) 1/16
D) 1/32
E) 1/64

E) 1/64

A black guinea pig crossed with an albino guinea pig produced twelve
black offspring. When the albino was crossed with a second black
animal, six blacks and six albinos were obtained. What is the best
explanation for this genetic situation?
A) Albino is recessive; black is dominant
B) Albino is recessie; black is codominant
C) Albino and black are codominant
D) Albino is dominant; black is incompletely dominant

A) Albino is recessive; black is dominant

Gray seed color in peas is dominant to white. Assume that Mendel
conducted a series of experiments where plants with gray seeds were
crossed among themselves, and the following progeny were produced: 302
gray and 98 white.
(i) What is the probable genotype of each parent?
(ii) Based on your answer, what genotypic and phenotypic ratios
are expected in these progeny? G= gray and g=white
A) Gg x Gg; genotypic= 1:2:1, phenotypic = 3:1
B) gg x Gg; genotypic = 1:2, phenotypic = 3:1
C) GG x gg; genotypic = 3:1, phenotypic= 1:2:1
D) GG x Gg; genotypic = 1:2:1, phenotypic = 2:1

A) Gg x Gg; genotypic= 1:2:1, phenotypic = 3:1

In a particular plant, leaf color is controlled by gene locus D.
Plants with at least one allele D have dark green leaves, and plants
with the homozygous recessive dd genotype have light green leaves. A
true-breeding dark-leaved plant is crossed with a light-leaved one,
and the F? offspring is allowed to self-pollinate. The predicted
outcome of the F? is diagrammed in the Punnett square shown in Figure
14.1, where 1, 2, 3, and 4 represent the genotypes corresponding to
each box within the square.
Which of the boxes correspond to plants with a heterozygous genotype?
A) 1
B) 1 and 2
C) 1, 2, 3 and 4
D) 2 and 3

D) 2 and 3

Which of the plants will be true-breeding?
A) 1 and 4 only
B) 2 and 3 only
C) 1, 2, 3, and 4
D) 1 only
E) 1 and 2 only

A) 1 and 4 only

Skin color in a certain species of fish is inherited via a single
gene with four different alleles. How many different
types of gametes would be possible in this system?
A) 1
B) 2
C) 4
D) 8
E) 16

C) 4

Mendel's observation of the segregation of alleles in gamete
formation has its basis in which of the following phases of cell division?
A) prophase I of meiosis
B) anaphase II of meiosis
C) metaphase I of meiosis
D) anaphase I of meiosis
E) anaphase of mitosis

D) anaphase I of meiosis

What was the most significant conclusion that Gregor Mendel drew from
his experiments with pea plants? A) There is
considerable genetic variation in garden peas.
B) Traits are inherited in discrete units, and are not the results
of "blending."
C) Recessive genes occur more frequently in the F? generation than
do dominant ones.
D) Genes are composed of DNA.
E) An organism that is homozygous for many recessive traits is at
a disadvantage.

B) Traits are inherited in discrete units, and are not the results of "blending.

The individual with genotype AaBbCCDdEE can make many kinds of
gametes. Which of the following is the major reason?
A) segregation of maternal and paternal alleles
B) recurrent mutations forming new alleles
C) crossing over during prophase I
D) different possible assortment of chromosomes into gametes
E) the tendency for dominant alleles to segregate together

C) crossing over during prophase I

Why did Mendel continue some of his experiments to the F? or F?
generation? A) to obtain a larger number of offspring on
which to base statistics
B) to observe whether or not a recessive trait would reappear
C) to observe whether or not the dominant trait would reappear
D) to distinguish which alleles were segregating
E) to be able to describe the frequency of recombination

B) to observe whether or not a recessive trait would reappear

Which of the following about independent assortment and segregation
is correct?
A) The law of independent assortment requires describing two or
more genes relative to one another.
B) The law of segregation requires describing two or more genes
relative to one another.
C) The law of segregation requires having two or more generations
to describe.
D) The law of independent assortment is accounted for by
observations of prophase I.
E) The law of segregation is accounted for by anaphase of mitosis.

A) The law of independent assortment requires describing two or more
genes relative to one another.

A sexually reproducing animal has two unlinked genes, one for head
shape (H) and one for tail length (T). Its genotype is HhTt. Which of
the following genotypes is possible in a gamete from this organism?
A) tt
B) Hh
C) HhTt
D) T
E) HT

E) HT

An original section of DNA has the base sequence AGCGTTACCGT. A
mutation in this DNA strand results in the base sequence AGGCGTTACCGT.
This change represents _____.
A) A silent mutation
B) A frameshift mutation
C) A point mutation
D) A missense mutation

B) A frameshift mutation

A single base substitution mutation is least likely to be deleterious
when the base change results in ____.
A) A codon that specifies the same amino acid as the original codon
B) A stop codon
C) An amino acid substitution that alters the tertiary structure
of the protein
D) An amino acid substitution at the active site of an enzyme

A) A codon that specifies the same amino acid as the original codon

Rank the following one-base point mutations (from most likely to
least likely) with respect to their likelihood of affecting the
structure of the corresponding polypeptide.
1. insertion mutation deep within an intron
2. substitution mutation at the third position of an exonic codon
3. substitution mutation at the second position of an exonic codon
4. deletion mutation within the first exon of the gene
A) 2,1,4,3
B) 1,2,3,4
C) 3,1,4,2
D) 4,3,2,1

D) 4,3,2,1

Of the following, which is the most current description of a gene?
A) A unit of heredity that causes formation of a phenotypic characteristic
B) A DNA subunit that codes for a single complete protein
C) a discrete unit of hereditary information that consists of a
sequence of amino acids
D) A DNA sequence that is expressed to form a functional product:
either RNA or polypeptide

D) A DNA sequence that is expressed to form a functional product:
either RNA or polypeptide

Which of the following types of mutation, resulting in an error in
the mRNA just after the AUG start of translation, is likely to have
the most serious effect on the polypeptide product?
A) a substitution of the first nucleotide of the GGG codon
B) a substitution of the third nucleotide in an ACC codon
C) a deletion of two nucleotides
D) a deletion of a codon

C) a deletion of two nucleotides

A nonsense mutation in a gene ____.
A) has no effect not he amino acid sequence of the encoded protein
B) introduces a premature stop codon into the mRNA
C) alters the reading frame of the mRNA
D) changes an amino acid in the encoded protein

B) introduces a premature stop codon into the mRNA

Which of the following DNA mutations is most likely to damage the
protein it specifies?
A) a codon deletion
B) an addition of three nucleotides
C) a base-pair deletion
D) a substitution in the last base of a codon

C) a base-pair deletion