Fermentation patterns are good in differentiating between what bacteria?
Gram negative bacilli, especially those that are members of Enterobacteriacae
Fermentation
Is a metabolic pathway in which carbohydrates are catabolized and small amounts of ATP are released
Is a metabolic process performed by many types of bacteria. Fermentation allows the production of ATP without the need for atmospheric oxygen.
How does fermentation differ from respiration?
1. Fermentation only involves glycolysis
2. ATP is created through substrate level phosphorylation
3. an organic molecule serves ad an electron donor and one or more of its endogenous organic products serves as the final electron acceptor
Phenol-Red broth
It is used in Carbohydrate Hydrolysis
It is a pH indicator that is red at neutral and yellow at pH< 6.4
Durham Tube
It is placed in the phenol red broth( inverted)
it traps any gas that is given off
What gas is given off during fermentation?
CO2 and H2
What are the steps in fermentation?
Carbohydrate-> glucose-> pyruvate-> fermentation by-products
Reversion of phenol-red broth
This happenes the the organism ferments all the available carbohydrate and switches to de-ammination of amino acids cause the tubes to start turning red. Meaning pH will raise
Acid Positive/negative results
(+)- tube turns yellow due to the presence of pyruvic acid as well as fermentation by-products
(-)- tube stays red
Gas Positive/ Negative results
(+)- durham tube has a defined air bubble at the top
(-)- Durham tube has small bubbles at the top. not one big defined ones
Why can bacteria ferment glucose and not sucrose?
Even though sucrose is a disaccharide composed of glucose and fructose, a bacteria is not able to break it down because it does not have the enzyme sucrase to break down sucrose in to fructose and glucose
What are the three was in which microorganisms can break down carbohydrates?
Aerobic and anaerobic respiration
Fermentation