Describe three points about biofilms that are beneficial to the bacteria who are present in them
1. Resistant to cell loss- cells in a biofilm are tightly stuck together, the shearing force of blood urine or other fluids cannot detach the cells
2. Resistant to antibiotics- cells in the interior of a biofilm are inaccessible to antibiotics and/or meta
Describe the colonisation sites of
- P.aeruginosa
- Stretococcus mutans
- Staph epidermidis
- Staph aureus
P.aeruginosa: Colonisation of the lungs of cystic fibrosis patients
- Strep.mutants: tooth surfaces
- S.epidermidis: colonisation of medical implants
- S.aureus- colonisation of medical implants
How do S.aureus cells adhere to the surface?
- Indirect association with host cells (bridging)
- Facilitated by FnBPs
What is the role of FnBPs in the attachment stage?
- Fibronectin in the blood coats the surface of medical implants
- FnBP on the surface of S.aureus mediates the binding of bacteria to the fibronectin coating the medical implant
What is the role of PIA in maturation?
- Polysaccharide intracellular adhesin is a polymer comprised of N acetyl glucosamine
- PIA forms an extracellular matrix that causes bacteria in a biofilm to stick together
What is the role of PSMs?
- They are a class of amphipathic peptides made by S aureus and S epidermidis
- PSMs become expressed after the maturation of biofilms. PSM expression is under the control of the Agr (accessory gene regulator) "quorum sensing" system
- PSMs promote struct
Describe three points about biofilms that are beneficial to the bacteria who are present in them
1. Resistant to cell loss- cells in a biofilm are tightly stuck together, the shearing force of blood urine or other fluids cannot detach the cells
2. Resistant to antibiotics- cells in the interior of a biofilm are inaccessible to antibiotics and/or meta
Describe the colonisation sites of
- P.aeruginosa
- Stretococcus mutans
- Staph epidermidis
- Staph aureus
P.aeruginosa: Colonisation of the lungs of cystic fibrosis patients
- Strep.mutants: tooth surfaces
- S.epidermidis: colonisation of medical implants
- S.aureus- colonisation of medical implants
How do S.aureus cells adhere to the surface?
- Indirect association with host cells (bridging)
- Facilitated by FnBPs
What is the role of FnBPs in the attachment stage?
- Fibronectin in the blood coats the surface of medical implants
- FnBP on the surface of S.aureus mediates the binding of bacteria to the fibronectin coating the medical implant
What is the role of PIA in maturation?
- Polysaccharide intracellular adhesin is a polymer comprised of N acetyl glucosamine
- PIA forms an extracellular matrix that causes bacteria in a biofilm to stick together
What is the role of PSMs?
- They are a class of amphipathic peptides made by S aureus and S epidermidis
- PSMs become expressed after the maturation of biofilms. PSM expression is under the control of the Agr (accessory gene regulator) "quorum sensing" system
- PSMs promote struct