Chapter 9: Microbial Genetics

Genome

Sum total of genetic material of an organism

Plasmids and circular chromosomes

Bacterial genes consist of

Chromosome in nucleus, mitochondria, and chloroplasts

Eukaryotic genes consist of

Genotype

Sum of all genes

Phenotype

Outward, visible expression of gene

Semiconservative

DNA replication is_____ meaning is consist of 1 parental strand and 1 daughter strand

Transcription

From DNA to RNA (mRNA)

Translation

From mRNA to proteins which occurs in ribosomes

Inducible Operon

-Operon turned on by substrate of enzyme for which structural genes code
-Enzyme needed to metabolize nutrient are only produced when nutrient present

Repressible Operon

-Normal state is "on"
-Often contain genes for anabolic enzymes
-Several genes are turned off by product synthesized by enzyme

DNA released from a killed cell can be acquired by a live cell

Griffith's experiment proved what?

Conjugation

Requires attachment of two related species and the formation of a cellular bridge that can transport DNA

Transformation

Entails transfer of naked DNA and requires no special vehicle

Transduction

DNA transfer mediated through the action of a bacterial virus