Causative agen of Lyme Disease
B. Burgdorferi
Method for Diagnosing Relapsing Fever
Direct Mircrosopic Observation
Method for Diagnosing Lyme Disease
Indirect Immunofluorescence
Spirochetes of T pallidum
6-20 micro Meter 8-15 spirals
Etiological aagent of Vernereal Syphilis
T. Palidum sub species Pallidum
Secondary syphilis, most logical place to isolate T.Pallidum
Skin lesions that make up rash
Spirochetes of Leptospira
6-12 micrometers 12-18 spirals
Leptospira 1/3 terminal is...
flexible and hook shaped
Serotype of Leptospira that causes Weil's disease, jaudice
Leptospira interrogans sevrovar ICTOHAEMORRHAgiae
Most usful strain specific test / reference method for Leptospirosis
Micro Aggultination test
Mollicutes are very particulat for proper growth require...
Cholesterol and yeast extract nucleic acid precursors
Since mollicutes dont have a cell wall they are resistant to...
beta-lactam
Mycoplasma species is associated with NGU
M. genitalium.
A7 agar and under ideal atmospheric conditions Mycoplasma produces what
characteristic colony appearance
Fried-egg
microorganism is associated with stillborn infants and shows a correlation between
colonization and fetal morbidity
U. urealyticum
Chlamydiae are obligate intracellular organisms referred to as
energy parasites, because they use the ATP produced by the host cell for their own
requirements
Which one of the following is not a method for laboratory diagnosis of chlamydial
infections
Growth in liquid medium supplemented with 10% rabbit serum
The following procedures are used in the routine diagnosis of rickettsial infections except
cultivation
The Weil-Felix serologic method is based on the fact that Rickettsia share antigens with
Proteus spp
Weil-Felix test can be used to diagnose which of the following
Typhus, scrub typhus, and Rocky Mountain spotted fever
nucleic acid genome of a virus is surrounded by a protein coat called
a capsid
Viruses lack enzymes and are unable to replicate outside the host cell, for this reason they
are referred to as
obligate intracellular parasites
Antiviral agents interfere with the virus' ability to
enter the cell
What is the standard by which all virology procedures are measured
Isolation and identification in cell culture
Viral infections elicit immune responses directed against which antigens
Core proteins, envelope proteins, glycoprotein spikes
Why is it necessary to obtain paired serum specimens two or three weeks apart in
diagnosing a viral infection
Antibodies are usually not formed during the early phase of the disease, and the first specimen
provides a base line for the second in testing antibody levels
Most specimens for viral analysis should be frozen at a temperature of
-70�C
When shipping specimens for the isolation of cytomegalovirus and varicella virus, the
contents should be
rapidly frozen
Safety in the mycobacteriology laboratory is important because many of the techniques
employed have the potential for creating hazardous aerosols of infectious
droplet nuclei
As a safety measure, what solution is the disinfectant for cleaning the laboratory following
mycobacteria processing
0.5 percent sodium hypochlorite
What biosafety level practices are required for labs performing identification and
susceptibility testing of mycobacterial isolates?
Biosafety level 3
Direct smears of feces for AFB is especially useful for evaluating what mycobacteria AIDS
patients
MAC
What is the second most common specimen encountered in the laboratory for diagnosis of
mycobacterioses?
Urine
Which of the following are the most popular egg-based media and agar-based media for
recovery of mycobacteria
L-J and Middlebrook 7H10 and 7H11
The only difference in the modified Kinyoun procedure from the Ziehl-Neelsen is the
mordant
Which is the most commonly used fluorochrome stain for identification of mycobacteria on
a smear
Auramine O-rodamine
The Mycobacterium are
aerobic, nonspore-forming, nonmotile bacilli.
chronic granulomatous (Hansen's) disease?
M. leprae
Which one of the following is not a method of identifying most mycobacterial pathogens
Growth at 25�C
Rapidly growing Mycobacterium usually mature within
7 Days
Which one of the following is not a simple fundamental structure of the fungi
Rhizoids
The vegetative phase of the fungi is characterized by
elongated, branching filaments
The two specified environmental conditions that could activate the reproductive phase of
the fungi life cycle are
temperature variations and lack of nourishment
Which of the following classes of true fungi include the rusts, smuts, and poisonous fleshy
mushrooms
Basidiomycetes
What class of fungi contains most of the fungi pathogenic to man
Deuteromycetes
Normally, fungi grow best around what acid pH
4.5/5.5
Differentiation of yeast and various species of Candida, identification of C. albicans can be
accomplished using
cornmeal agar with Tween 80
Systemic mycoses are deep-seated infections that may spread throughout the body but
primarily begin in the
lung
The following are advantages of culture tubes over Petri dishes for fungus cultures except
better aeration of the cultures
Macroscopic examination of fungus growth characteristics includes but is not limited to
rate of growth, texture, and pigmentation
Probably the most widely used of all wet mounts is
potassium hydroxide
Before collecting skin scrapings for a KOH mount, the lesion area is thoroughly washed
remove dirt particles, bacteria, and medications
The India ink wet mount reveals the encapsulated budding or non-budding blastospores of
Cryptococcus neoformans