Microbiology Exam #2

which process 'copies the DNA strands':

replication

which of these involves transfer of DNA in solution:

transformation

which of these would turn on an inducible enzyme like 'lac':

inducer/repressor

which of these methods of sample preparation would favor anaerobic microbes:

use a CO2 chamber

which of these methods sterilizes with sound vibration:

sonnication

which of these uses organic molecules as both energy source and carbon source:

chemoheterotrophs

which of these would most likely produce alcohols or organic acids (and gas) as end products:

fermenters

photosynthesis involves all of these except:

biodegradation

the photosynthetic process used by photoautotrophs requires all of these 'raw materials' except:

glucose

light driven photosynthesis may form any of these except:

dioxin

which of these is the main pigment used in the process of photosynthesis:

chlorophyll

pili tubes move genetic information from donor to recipient:

conjugation

an example of a fermenting microbe is:

escherichia

energy in biological systems is primarily:

chemical

for mutations to have an effect on populations of microbes:

only a & b

the process of DNA rewriting information into RNAs is called:

transcription

the process of RNA codons being interpreted is called:

translation

the process of ribosomes reading coded messages and assembling proteins is called:

translation

three types of RNA may be formed from DNA templates; which of these is NOT a type of RNA:

xRNA

how many different kinds of amino acids many be used to synthesize the various proteins that compose living cells:

20

which characteristic is not true of plasmids:

required for cell function

the PCR reaction requires all of these except:

electrophoresis

the function of ligase is to

rejoin segments of DNA

which of these are important in cloning an isolated gene:

all of these

which gene is incorporated in plasmids to detect recombinants:

antibiotic resistance

a pathogen of plant roots that is used as a cloning host is:

agrobacterium

which of the following are pyrogenic cocci:

streptococcus, staphylococcus, neisseria (all of these)

the skin blotches in meningitis are due to:

endotoxins in blood

rheumatic fever damages the ___, and glomerulonephritis damages the _____.

heart valves, kidneys

what is the usual habitat of endospore forming pathogens:

dust and soil

the action of tetanus endotoxin is on the:

spinal interneurons

TB is spread by:

respiratory droplets

which of these infections is characterized as zoonotic:

anthrax

a unique visible trait of many variants of pseudomonas is:

fluorescent pigment

haemophilus influenzae requires ___ for growth:

blood

the severe symptoms of pertussis are due to:

killed respiratory epithelia

shigella is transmitted by:

food, flies, feces (all of these)

which of these is produced by streptococcus and dissolves clots:

streptolysin, streptokinase, pyrogenic toxin

which of these gram negative microbes causes whooping cough:

bordatella

which of these bacteria may cause ulcers:

heliobacter

jacob and monod named the entire unit for expressing a particular gene an OPERON. (T OR F)

T

when an inducer nutrient is absent from the cytoplasmic environment, the repressor gene codes for mRNA and repressor (T OR F)

T

in the 'lac operon', inducer molecules bind preferentially with repressor protein forms (T OR F)

T

scientists are learning to re- engineer bacteria to produce useful materials (T OR F)

T

cyanobacteria practice photosynthesis in a manner similar to eukaryotes like grass and maple trees (T OR F)

T

A control mechanism for protein synthesis is illustrated by the operon theory (T OR F)

T

when ATP forms aerobically, Krebs cycle enzymes are operating (T OR F)

T

some microorganisms contain extrachromosomal DNA in the form of plasmids (T OR F)

T

plasmids may code for antibiotic resistances or other selective features within cells (T OR F)

T

all mutations are induced, NONE are spontaneous (T OR F)

F

arthropod vectors of disease include mosquitos and ticks (T OR F)

T

mycoplasmas attack the lung cell membranes of hosts (T OR F)

T

treponema pallidum produces the gummas of syphillis (T OR F)

T

lyme disease symptoms include rash, arthritis, and aches (T OR F)

T

dental caries (cavities) are never produces by microbes (T OR F)

F

number of energy molecules needed to start glycolysis:

two

proton pumps in the ETS makes this universal energy:

ATP

reducing power molecules formed during glycolysis and krebs cycle:

FADH and NADH

molecule that recharges the krebs cycle (2 carbons)

acetyl coA

total # of energy molecules recovered from one glucose that has been broken down aerobically

36-38

co- enzymes and enzymes are made from these micronutrients

B- complex and vitamin

organic molecule produced anaerobically from pyruvate

ethanol

activated (phosphorylated) glucose is split into two molecules (glycolysis)

GAP and DHAP

the nitrogenous bases of DNA, A, T, G, C are bonded to:

deoxyribose

the DNA base pairs are held together by:

hydrogen bonds

which of these 'powers' electron transport systems (ETS)

proton pumps

which of these would not be a product of photosynthesis:

nitrites

which of these uses light as an energy source and organic compounds as a carbon source (purple nonsulfure & green nonsulfur bacterial):

photoheterotrophs

anabolism of proteins is also known as (opp. of catabolism):

protein synthesis

name one bacterial STD and the germ that causes it:

gonorrhea and neisseria gonorrhea

name one soil or arthopod borne bacterial disease and the specific microbe (NOT THE VECTOR) that causes it:

Rocky Mountain Spotted Fever and Rickettsia rickettsii

name one food or waterborne bacterial diseases and its germ

salmonellosis and salmonella enterica

name one respiratory tract bacterial diseases and its germ

whooping cough and bordatella pertussis

glucose is activated with phosphates, cleaved to DHAP and GAP, forms pyruvate

glycolysis

DNA codes are written into mRNA codons

transcription

rRNA reads message and assembles specific protein at ribosome site

translation

electron transport system; chemiosmosis proton motive force operating

proton pumps

DNA copies itself matching base pairs

replication

acetyl coA combines with OAA and cycles to makes a lot of NADH and FADH

krebs cycle

which process is part of protein synthesis:

transcription

which of these involves transfer of genes via pilli:

conjugation

inducible enzymes:

are proteins, turn- on when needed, form I/R complex (all of these)

which of these methods ensures complete sterilization:

autoclave

which of these methods uses penetrating shortwaves:

UV light

which of these uses inorganic molecules for energy and carbon source:

...

which process 'copies the DNA strands':

replication

which of these involves transfer of DNA in solution:

transformation

which of these would turn on an inducible enzyme like 'lac':

inducer/repressor

which of these methods of sample preparation would favor anaerobic microbes:

use a CO2 chamber

which of these methods sterilizes with sound vibration:

sonnication

which of these uses organic molecules as both energy source and carbon source:

chemoheterotrophs

which of these would most likely produce alcohols or organic acids (and gas) as end products:

fermenters

photosynthesis involves all of these except:

biodegradation

the photosynthetic process used by photoautotrophs requires all of these 'raw materials' except:

glucose

light driven photosynthesis may form any of these except:

dioxin

which of these is the main pigment used in the process of photosynthesis:

chlorophyll

pili tubes move genetic information from donor to recipient:

conjugation

an example of a fermenting microbe is:

escherichia

energy in biological systems is primarily:

chemical

for mutations to have an effect on populations of microbes:

only a & b

the process of DNA rewriting information into RNAs is called:

transcription

the process of RNA codons being interpreted is called:

translation

the process of ribosomes reading coded messages and assembling proteins is called:

translation

three types of RNA may be formed from DNA templates; which of these is NOT a type of RNA:

xRNA

how many different kinds of amino acids many be used to synthesize the various proteins that compose living cells:

20

which characteristic is not true of plasmids:

required for cell function

the PCR reaction requires all of these except:

electrophoresis

the function of ligase is to

rejoin segments of DNA

which of these are important in cloning an isolated gene:

all of these

which gene is incorporated in plasmids to detect recombinants:

antibiotic resistance

a pathogen of plant roots that is used as a cloning host is:

agrobacterium

which of the following are pyrogenic cocci:

streptococcus, staphylococcus, neisseria (all of these)

the skin blotches in meningitis are due to:

endotoxins in blood

rheumatic fever damages the ___, and glomerulonephritis damages the _____.

heart valves, kidneys

what is the usual habitat of endospore forming pathogens:

dust and soil

the action of tetanus endotoxin is on the:

spinal interneurons

TB is spread by:

respiratory droplets

which of these infections is characterized as zoonotic:

anthrax

a unique visible trait of many variants of pseudomonas is:

fluorescent pigment

haemophilus influenzae requires ___ for growth:

blood

the severe symptoms of pertussis are due to:

killed respiratory epithelia

shigella is transmitted by:

food, flies, feces (all of these)

which of these is produced by streptococcus and dissolves clots:

streptolysin, streptokinase, pyrogenic toxin

which of these gram negative microbes causes whooping cough:

bordatella

which of these bacteria may cause ulcers:

heliobacter

jacob and monod named the entire unit for expressing a particular gene an OPERON. (T OR F)

T

when an inducer nutrient is absent from the cytoplasmic environment, the repressor gene codes for mRNA and repressor (T OR F)

T

in the 'lac operon', inducer molecules bind preferentially with repressor protein forms (T OR F)

T

scientists are learning to re- engineer bacteria to produce useful materials (T OR F)

T

cyanobacteria practice photosynthesis in a manner similar to eukaryotes like grass and maple trees (T OR F)

T

A control mechanism for protein synthesis is illustrated by the operon theory (T OR F)

T

when ATP forms aerobically, Krebs cycle enzymes are operating (T OR F)

T

some microorganisms contain extrachromosomal DNA in the form of plasmids (T OR F)

T

plasmids may code for antibiotic resistances or other selective features within cells (T OR F)

T

all mutations are induced, NONE are spontaneous (T OR F)

F

arthropod vectors of disease include mosquitos and ticks (T OR F)

T

mycoplasmas attack the lung cell membranes of hosts (T OR F)

T

treponema pallidum produces the gummas of syphillis (T OR F)

T

lyme disease symptoms include rash, arthritis, and aches (T OR F)

T

dental caries (cavities) are never produces by microbes (T OR F)

F

number of energy molecules needed to start glycolysis:

two

proton pumps in the ETS makes this universal energy:

ATP

reducing power molecules formed during glycolysis and krebs cycle:

FADH and NADH

molecule that recharges the krebs cycle (2 carbons)

acetyl coA

total # of energy molecules recovered from one glucose that has been broken down aerobically

36-38

co- enzymes and enzymes are made from these micronutrients

B- complex and vitamin

organic molecule produced anaerobically from pyruvate

ethanol

activated (phosphorylated) glucose is split into two molecules (glycolysis)

GAP and DHAP

the nitrogenous bases of DNA, A, T, G, C are bonded to:

deoxyribose

the DNA base pairs are held together by:

hydrogen bonds

which of these 'powers' electron transport systems (ETS)

proton pumps

which of these would not be a product of photosynthesis:

nitrites

which of these uses light as an energy source and organic compounds as a carbon source (purple nonsulfure & green nonsulfur bacterial):

photoheterotrophs

anabolism of proteins is also known as (opp. of catabolism):

protein synthesis

name one bacterial STD and the germ that causes it:

gonorrhea and neisseria gonorrhea

name one soil or arthopod borne bacterial disease and the specific microbe (NOT THE VECTOR) that causes it:

Rocky Mountain Spotted Fever and Rickettsia rickettsii

name one food or waterborne bacterial diseases and its germ

salmonellosis and salmonella enterica

name one respiratory tract bacterial diseases and its germ

whooping cough and bordatella pertussis

glucose is activated with phosphates, cleaved to DHAP and GAP, forms pyruvate

glycolysis

DNA codes are written into mRNA codons

transcription

rRNA reads message and assembles specific protein at ribosome site

translation

electron transport system; chemiosmosis proton motive force operating

proton pumps

DNA copies itself matching base pairs

replication

acetyl coA combines with OAA and cycles to makes a lot of NADH and FADH

krebs cycle

which process is part of protein synthesis:

transcription

which of these involves transfer of genes via pilli:

conjugation

inducible enzymes:

are proteins, turn- on when needed, form I/R complex (all of these)

which of these methods ensures complete sterilization:

autoclave

which of these methods uses penetrating shortwaves:

UV light

which of these uses inorganic molecules for energy and carbon source:

...