EEMB 111 Lab Pratical Final

Emerita Analoga

Sand Crab (dissection) - look for cystacanths

Blephariopoda Occidentalis

mole crab

Phylum: Arthopoda

Subphylum - crustacea
subclass - copepod
-diverse free living and symbiotic
-mostly marine, some fresh water

Subclass - Branchiura

-two adhesive disks and piecing stylet
-ectoparasite of fishes

Subclass - Pentastomida

-highly modified arthropods
-tropically transmitted
-complex L.C.
Respiratory tract of DH

Subclass - thecostraca

super order- Thoracica
-typical barnacle -->whale

Super order - Rhizocephala

-"Root Head"
-mostly endoparasites of crustaceas
-castrators
-behavior mod

Pugettia producta

-LIVE DEMO
-kelp crab infected with heterosacchus californicus
-remember what the infected sac looks like**

Phylum - arthropoda

subphylum - crustacea
Class - malacostraca
-crabs, isopods, decapods, etc.

Order- Amphipoda

-mostly free living

Pathogen

-reproduces on host, contact between whales need for transmission (mom --> calf; mating)

Order - isopoda

Superfamily - cyanothoidea
Family - Cymothoidae
- gills and body surface
- can be FACULTATIVE CASTRATORS
- Infective stage LARVAE --> Manca Larvae

Family - Bopyridae

-look for in PACHYCHELES --> crab
-two host L.C.
-infective stage larval
-Epicardium larva infects copepod (IH)
-Cyptoniscus larval infects decapod (DH)

Facultative Castrator

-feed on hemolymph can suppress host reproductive

Family - entoniscidae

-look in Hemigraspus Oregonesis --> small shore crab
-two host LC with epicardium and cytoniscus
-Females - vestigial limbs, loss of exoskeleton

Class - insecta

-ectoparasites
-pathogens
-micropredators
-vectors
-parasitoid
-3 body segments:
-head
-thorax
-abdomen

Order - phthiraptera

-sucking lice (Anoplura) vs. chewing lice (Ischnocera)

Anoplura

-sucking lice

Ischnocera

-chewing lice

Order - Siphonaptera

-fleas
-latterly compressed
-bubonic plage

Order - hemiptera

-true bugs
-piercing stylet
-Triatoma spp - kissing bugs
-Cimex spp - bed bugs

Triatoma

-kissing bugs
-CHAGAS disease

Cimex

Order- diptera

-flies, mosquitos, gnats
-Micro predators and vectors
-mosquitoes (culex, anopheles, )
--->malaria, Zika, West nile, Wuchera
- black flies (simulium) -->Onchocerca volvulus --> river blindeness
- Tse tse flies (Glossina) --> Sleeping sickness

Mosquitoes

-malaria, west nile,

Simulium

-onchocerca volvulus - river blindness
-black flies

Glossina

-tse tse flies
-sleeping sickness

Subclass - Acari

adults - 4 pairs of legs
nymphs - 3 pairs of legs

Family - Ixodidae

-ticks - vectors
-scutum
-Dermacentor anderson --> Rocky mountain spotted fever

Family - argasidae

-soft ticks
no secutum
-subterminal head

Amoebas - unranked (study the structures from LAB MAN)

-trophozoite and cyst stages
-cyst are diagnostic stage
-endocommensals and endoparasites

Porichthys notaus

-gallbladder
-myxozoa --> gallbladder

Flagellates

-informal group

cilia vs flagella

cilia
-beating motion
-covers entire cell
flagella
-propeller like motion
-longer usually few

Human Flagellates

-Chdomastix meslini
-Giardia lambia
-dientamoeba fragilis
-Trichomonas vaginalis

Chdomastix meslini

-mainly pigs
-4 flagella
-pyriform shape
-trophs in cecum/ colon, cyst feces -->water

Giardia lambia

-humans + mammals
-8 flagella
-binucleate
-adhesive disk
-trophs in intestine

Dientamoeba fragilis

-humans
-2 nuclei in troph***
-pesudoplasmodium - no flagella on troph
-troph in intestine, with no cyst stage
-possibly vectored by pin worms

Trichomonas vaginalis

-human
-5 flagella (4 anterior)
-undulating membrane
-trophs in urogenital tract
-no cyst
-STD strong team defense

Other Flagellates

- opalina**

Host - Pseduacris regilla ** disection --Pacific tree frog

-appears ciliated, but are evolutionary close to flagella
-multinucleate

Order - kinetoplastida

-flagellum
-single, large mitochondria
-TROPHS only

Kinetoplast

-granule with in mitochondrion of base of flagellum (stains dark)

Kinetosome

-structure where flagellym arises

Four forms of trophs - study in LAB MAN

-Amastigote
-promastigote
-epimastigote
-trypomastigote

Leishmania

-species
-vector-->sand fly (phlebofomes)
-Promastigote in fly
-Amastigote in humans

Salivaria

-trypanosomes injected in host via salivary glands on vector

Stercoranria

-develop in insect gut, transmitted through feces

Trypanosoma Brucei (know the life cycles)

Salivaria group
Tse tse fly vector
-Brucei
-gambiense
-rhodesiense

T.B. Brucei

Host- livestock
Pathology - Nagana
Distribution - Africa
Morphs - trypomastigotes --> blood, major organs, cerebral spinal flood

T.B. Gambiense

Host- humans
Pathology - African Sleeping Sickness
Distribution - West central Africa
Morphs - trypomastigotes --> nervous system and brain
CHRONIC symtoms --> dullness, tremors, paralysis, and convulsions

T.B. rhodesiense

Host - humans
Pathology - African Sleeping sickness
Distribution - East Central Africa
Morphs - trypmastigotes --> most organs, rarely nervous system
ACUTE symptoms --> swelling of lymph nodes, fevers, weakness, cramping

Trypansoma (know the life cycles)

Stercoaraia group - vector shits on yo skin and you scratch dat shit in wound
-Cruzi
-Lewis

T. Cruzi

Vector - kissing bug (reduviidae)
Host - Humans
Pathology - Chagas disease
Distribution - south central Africa
Morphs - trypomastigotes, epimastigotes

T. Lewisi

Vector - rat flea (nosopsyllus faciatus)
Host - Rat
Pathology - mild
Distribution - world wide
Morphs - trypomastigotes

Leishman (know the life cycles)

Vector - sandfly (Phlebotomus)
Host - human, dogs, rodents
Pathology - viceral cutaneous
Distribution - world wide
Morphs - Promastigote(insect vector) and amastigote(vertebrate)

Phylum: ciliophora

-trophozoites - feeding stage
-cyst - produces tomites
-tomites - motile, infective stage

Balantidium Coli

-pigs and other mammals
-pathology - dysentery (blood in shit)

Ichthyopthirius multifilii's

-"ich"
-freshwater fish
-tomites infective

trichodina

-fish, amphibian and aquatic invets
-low path
-DENTICLES

Phylum - apicomplexa

Class - conoidasida
subclass - gregarina

Monocystis lumbrici

-earth worm: lumbricus terrestralis
-

Phylum - Apicomplexa

Class - coccidea

Family - Emieridae

-direct lc
-oocyst 1:4:2 config
-each oocyst contains 4 sporocyst each containing 2 sporozoites

Eimeria Tenella

-intestine of chickens
-severe pathology, destroys intestinal tissue

Eimeria stidae

-in bile ducts and liver of rabbits
-liver failure

Family - sarcocystidae

-oocyst 1:2:4
-each oocyst contains 2 sporocyts each with 4 sporozoites
-IH tachyzoites (fast reproduction merozoites) Bradyzoites (large merozoites, Trophically transmitted to DH)

Toxoplasma gondii

-DH cats
-IH many, humans
-DH infected by sporulated oocyst or bradyzoites

Sarcocysts

-DH carnivores
-IH herbivores
DH- only infected by bradyzoites

Phylum - apicomlexa

Class - coccidea
Order - haemosporida

Life cycle

-merogony(shizongony) -vertebrate host
-gamogony - initiated in vertebrate host, finalized in invertebrate host
-fertilization/zygote formation: in invertebrate vector
-sporogony - invertebrate vector

Human Malaria

- vectored by anopheles
-plasmodium vivax
-plasmodium falciparum
-plasmodium malaria
-Plasmodium ovale

Plasmodium vivax

-43% of cases, more temprate distibution, mostly South America
-large ring trophs
-Shuffners dots
-Mergony 48hrs

Plasmodium - falciparum

-50%, most virulent and prevalent
-small ring trophs -->mult per RBC as possible
-Asynchronous merogony approx 48 hrs
-black urine --> lysed RBC

Plasmodium malaria

-7% of cases
-similar in appearance to Pl vivax
- no shuffners dots
-merogony - 72hrs
Trophys have many band shapes

Plasmodium ovale

-rarest form, most mild symptoms
-shuffners dots

Avian Malria

-vectored by culex --> mosquito

Order- Piroplasmida

-vectored by ticks
-are very small and tear dropped shaped
-Babesia bigmania- in red blood cells of cattle (Texas Cattle fever)
-Thelaria parva - cattle (east coast fever)