* T-Soy Agar
This will be used for the gram stain of the bacteria, as well as to perform a Catalase Test on the unknown Bacteria.
Catalase Test: Reaction that turns Hydrogen Peroxide into water & O2.
*REGAGENT NEEDED: HYDROGEN PEROXIDE. Add Reagent to plate, if BUBBLE
Phenol Red Test: Gas Production
This test looks for TWO things: The Fermentation of sugar, and the presence of trapped gas. The Gas traps in a smaller Test tube inside the regular one called a "Durham Tube."
- The presence of BUBBLES in the Durham Tube indicates a POSITIVE Result for Ga
Phenol Red Test: Fermentation of Sugar
The broth in this contains Phenol Red, a pH indicator. A pH below 6.4 will turn the Phenol Red to Yellow. During fermentation, acids are produced that lower pH. You CAN have one result without the other, or both as the same result (Gas and Fermentation).
* Tryptophamase Test
This test determines the presence of the enzyme that catalyzes the reaction that breaks down the amino acid Tryptophan into Indol, Pyruvic Acid, & Amonia.
These are used as nutrients by the orgainism, except for Indol, which will remain behind. Detecting
Citrase Utlization Test
This is a Green Test Tube. This test determines if an organism uses the substrate Citrate. It needs the enzyme citrate permease to utilize the citrate. This test tube contains a pH indicator called Brum Thymol Blue, which is green in a neutral pH. A rise
Motility Test
This test determines the ability of an organism to be mobile. A stab of a bacteria into a test tube was performed:
- If you can CLEARLY see where the stab is, the organism is NON MOTILE
- If you can NOT clearly see where the stab is, the organism is consi
* Methyl Red Test
This is used to determine if the organism is capable of Mixed Acid Fermentation. Some organisms can process many acids (Fomic, Acedic, Lactic & Succinic), which will cause the pH to drop considerably.
*REAGENT NEEDED: Methyl Red (8 Drops). This reagent st
* Voges-Proskauer Test
This test determines if the organism will undergo alcohol fermentation. By products are produced during alcohol fermentation (2,3 bicarbonate & acetylmethol carbinol/acetoin). Detecting the presence of Acetoin will determine if the organism can undergo al
Kligers Iron Agar Test
The media contains the sugars Dextrose & Lactose.This test is to determine if the organism will utilize thiosulfate. The enzyme disulfurase allows for this utilization. An organism that has this enzyme will use sulfate to produce Hydrogen sulfide.
The med
Escherichia coli
Gram Reaction: Negative
Morphology: Bacilli
Arrangement: Single Rods
Enterobacter aerogenes
Gram Reaction: Negative
Morphology: Bacilli
Arrangement: Single
Salmonella typhimurium
Gram Reaction: Negative
Morphology: Bacilli
Arrangement: Pairs or Short Chains
Staphylococcus aureus
Gram Reaction: Positive
Morphology: Cocci
Arrangement: Clusters
Enterococcus faecalis
Gram Reaction: Positive
Morphology: Cocci
Arrangement: Pairs or Short Chains