KBCC Microbiology (BIO 51 - Dr. Lopez)

The four processes of life

Growth, reproduction, responsiveness, and metabolism

Growth

Living things can grow; that is, they can reproduce

Reproduction

Organisms increase in number, producing more like themselves

Responsiveness

Living things ability to respond to their environment

Metabolism

Organisms ability to take in nutrients and use them in a series of controlled, chemical reactions to provide the energy and structure needed to grow, reproduce, and be responsive. Cells store metabolic energy in the chemical bonds of adenosine triphosphat

First chemical similarity between Prokaryotes and Eukaryotes: "they both contain...

They both contain nucleic acid, proteins, lipids, and carbohydrates.

Second chemical similarity between prokaryotes and eukaryotes: "they use the same kinds of...

They use the same kinds of chemical reactions to metabolize food, build proteins, and store energy.

Prokaryote chief distinguishment: "DNA

Their DNA is not coated within a membrane and is one circular chromosome. Also their DNA is not associated with histones (special chromosome proteins found in eukaryotes)

Prokaryote chief distinguishment: "they lack...

They lack membrane enclosed organelles.

Prokaryote chief distinguishment: "their cell walls...

Their cell walls almost always contain the Complex polysaccharide peptidoglycan

Prokaryote chief distinguishment: "they divide by...

They divide by binary fission. During this process, the DNA is copied and cell splits into two cells.

Eukaryotes chief distinguishment: "DNA

Their DNA is found in the cells nucleus, which is separated from the cytoplasm by a nuclear membrane, and the DNA is found in multiple chromosomes. Also, their DNA is consistently associated with chromosomal proteins called Histones.

Eukaryotes chief distinguishment: "They have a number of...

They have a number of membrane-enclosed organelles, including mitochondria, endoplasmic reticulum, Golgi complex, lyosomes, and sometimes chloroplasts.

Eukaryotes chief distinguishment: "Their cell walls...

Their cell walls, when present, are chemically complex.

Eukaryotes chief distinguishment: "They usually divide by...(which is guided by)

They usually divide by mitosis, in which chromosomes replicate and an identical set is distributed into each of two nuclei. This process is guided by the mitotic spindle, an assembly of micro tubules. Division of the cytoplasm and other organelles follows

Prokaryotic Cells

They include bacteria and archaea. Although bacteria and archaea look similar, they are different in chemical composition. The membranes of this world make up a vast heterogeneous group of very small unicellular organisms.

Differentiating Factors of Bacteria

Morphology, Chemical composition, Nutritional requirements, Biochemical activities, and Source of energy.

Three common shapes of Bacteria

Coccus (berry), Bacillus (rod-like), and Spirial

Cocci

These are usually round, but can be oval. When cocci divide to reproduce, the cells can remain attached to one another.

Diplococci

Cocci that remain in pairs after dividing

Streptococci

Cocci that divide and remain attached in chain-like pairs

Tetrads

Cocci that divide in two planes and remain in groups of four.

Sarcinae

Cocci that divide in three planes and remain attached in cube-like groups of eight.

Staphylococci

Cocci that divide in multiple planes and form grape-like clusters.

Bacilli

These divide only across their short axis, thus there are fewer groupings of these then cocci. Most of them appear in single rods.

Diplobacilli

Bacilli that appear in pairs after division.

Streptobacilli

Bacilli that occur in chains.

Coccobacilli

Bacilli that look like cocci

Spiral

These bacteria have one or more twists; they are never straight.

Vibrios

Bacteria that look like curved rods.

Spirilla

Bacteria that have a helical shape, like a corkscrew, and fairly rigid bodies. These use whiplike extermal appendages called flagella to move.

Spirochetes

Another group of Bacteria spirals that are helical and flexible. These move by means of axial filaments, which are contained within a flexible, external sheath.

Additional shapes of Bacteria

Star shape, Rectangular, and Triangular.

What is shape of Bacteria determined by?

Heredity

Monomorphic

Bacteria that maintain a single shape. Genetically, bacteria are mostly this shape.

Pleomorphic

Bacteria that have a number of shapes. Rhizobium and Corynebacterium are examples.

External structures of Prokaryotic cell walls:

1.) Glycocalyx, 2.) Flagella, 3.) Axial filament, 4.) Fimbriae, 5.) Pili

Glycocalyx

The term means "sugar coat." it is a general term referring to substances that surround cells. The bacterial is a viscous (sticky), gelatinous polymer that is external to the cell wall and composed of polysaccharides, polypeptide, or both. Its chemical co

Slime Layer

if the substance is unorganized and only loosely attached to the cell wall, the glycocalyx is described as this.

What can a glycocalyx protect a cell against?

It can protect a cell against dehydration, and its viscosity may inhibit the movement of nutrients out of the cell.

Virulence

This term refers to the degree to which a pathogen causes disease.

Capsule

When the substance is organized firmly attached to the cell wall, a glycocalyx is described as this. These are important to contributing to virulence in bacteria. They often protect pathogenic bacteria from phagocytosis by cells of the host.

Three examples of Capsule containing bacteria.

1.) Bacillus Anthracis, 3.) Streptococcus Pneumoniae, 3.) Klebsiella Pneumoniae

Bacillus Anthracis capsule produced

D- glutamic acid. Because only encapsulated B. anthracis causes anthrax, it is speculated that the capsule may prevent it from being destroyed.

Streptococcus Pneumoniae capsule produced

Streptococcus causes pneumonia only when the cells are protected by a polysaccharide capsule. On the other hand, unencapsulated S. pneumoniae cells cannot cause pnemonia since they are readily phagocytized.

Klebsiella

The polysaccharide capsule that also prevents phagocytosis and allows the bacterium to adhere to and colonize the respiratory tract.

Extracellular polysaccharide (EPS)

A glycocalyx made of sugars. This enables a bacterium to attach to various surfaces in its natural environment in order to survive. Through attachment, bacteria can grow on diverse surfaces such as rocks in fast-moving streams, plant roots, human teeth, m