Simple stain
This slide was created by adding bacteria then heat fixing. After heat fixing a single stain was added then rinsed with water. What type of stain is this?
Negative stain
This slide was created by adding a drop of an acidic dye to a slide then adding bacteria with a loop. This mixture was then spread across the slide using a second slide. Once this slide dries it is ready for viewing. What type of stain is this?
Differential stain
This slide was created using two different stains, which allows for the detection of differences between organisms or even parts of the same organism. What type of stain is this?
Basic stain
This slide was created using a cationic or positively charged stain. The use of the positively charged stain allows it to bind the negatively charge cell wall, thus staining the cell. What type of stain is this?
Acidic stain
This slide was created using a negatively charged stain. Because the stain carries a negative charge it is repelled away from the negatively charged cell walls. What type of stain is this?
Gram stain
This slide was created using two different stains: crystal violet and safranin. This technique is used to differentiate between gram positive and gram negative bacteria. What type of stain is this?
Capsule stain
This stain was made using two different stains. This stain is used to detect cells capable of producing biofilm. What type of stain is this?
Endospore stain
This is a differential stain used to detect the presence and location of spores in bacterial cells. What type of stain is this?
Starch hydrolysis
The above test is used to determine what?
Starch agar
What type of agar is used in this test?
Positive
Would this be considered a positive or negative starch hydrolysis test?
Flagella stain
This stain is used to visualize flagella. In order to do so a mordant is used to encrust flagella with stain to a visible thickness. what type of stain is this?
Sugar fermentation test
The above test is used to determine sugar fermentation. What test is this?
Glucose, lactose and sucrose
The above test is used to determine sugar fermentation. It has the ability to test for the fermentation of three different sugars. Name these sugars:
gas production, Durham Tube
The above test has a glass vial inside the tube. What does this vial test for? What is it called?
Phenol Red
What indicator is used in the above sugar fermentation test?
The first yellow tube (A/-)
Which on of these tubes would be positive for sugar fermentation with no gas produced when running a sugar fermentation test?
The red tube furthest to the right (-/-)
Which one of these tubes would indicate no fermentation when running a sugar fermentation test?
The pink tube (K)
Which one of these tubes would indicate the organism is able to break down sugars into peptone when running a sugar fermentation test?
The second yellow tube (A/G)
Which one of these tubes would indicate that the organism is able to ferment sugar and also has a gas by product when running a starch hydrolysis test?
Motility
The above test is used to determine what?
The first and last tubes
Which of the above tube(s) would be considered positive for motility?
Motility test medium
What type of agar is used in the above test?
Catalase test
The above image is of what test?
The sample of the right
Which one of these would be considered a positive result for a catalase test?
H2O2, hydrogen peroxide
What reagent is used in the above test?
The organism grown on the plate produces the enzyme catalase
What does this test determine?
Oxidase test
The above image shows what test?
Positive
Would this be a positive or negative oxidase test?
Chromogenic reducing agent
What reagent is used in the above test?
Nitrate reduction test
The above test is used to determine whether an organism reduces nitrate to nitrite. What is this test called?
Tube 2
The above tubes have been incubated for 24 hours, after incubating all four tubes remained yellow with no bubble formation. Thus, 8 drops of reagent A and B were added to tubes 1 and 2. Which tube indicates a positive result, 1 or 2?
Tube 3
After adding reagent A and B to tubes 3 and 4 no color change was seen, both tubes remained yellow. Zinc power was then added, which tube would be considered a positive result tube 3 or 4?
Urease test
The above test is used to differentiate organisms on their ability to hydrolyze urea using the enzyme urease. What is this test called?
Phenol red
What is the indicator used in the urease test?
The pink tube (+)
Which on of the above tubes is positive for urea hydrolysis?
Both the yellow and orange tubes (-)
Which one of these tubes is negative for urease hydrolysis?
Antibiotic resistance and susceptibility
The above Kirby-Bauer test is used to determine what?
Mueller Hinton
What medium is used for the above Kirby-Bauer test?
The zone of inhibition
In order to test for susceptibility in the Kirby-Bauer test, what do you measure?
Staph aureus
The above image shows three different species of staph grown on MSA. What species is most likely growing in section 1?
Mannitol fermentation and the reduction of the pH
What caused section 1 of this plate to turn yellow?
Gram-negative organisms
Does the above MSA plate select for gram-positive of gram-negative organisms?
Staphylococcus
What organism does the above medium favor the growth of?
Coagulase test
The above test is used to determine whether an organism has the ability to clot. What is this test called?
The bottom tube
Which one of these tubes would be considered a positive result when running a coagulase test?
To differentiate S. aureus from other gram-positive cocci
Why would you run a coagulase test?
CAMP
The above image is an example of what test?
It can be used to identify Streptococcus agalactiae. Though not strongly beta-hemolytic on its own, it presents with a wedge-shape in the presence of Staphylococcus aureus.
What does the CAMP determine?
Beta hemolysis
The above organism is growing on blood agar, what type of hemolysis is seen?
Alpha hemolysis
The above organism is growing on blood agar, what type of hemolysis is seen?
Gamma hemolysis
The above organism is growing on blood agar, what type of hemolysis is seen?
Bacitracin
The above test is used to test susceptibility to what antibiotic?
Beta, streptococci, beta
Both of the organisms on the above plate display ________________ hemolysis. The bacitracin susceptibility test is often used to differentiate group A __________________ (strep pyogenes) from other _________________ hemolytic streptococci. Group A strepto
Blood agar
The above bacitracin susceptibility test is run on what medium?
Bile esculin
The above test is grown on what medium?
Positive, any darkening of the medium is considered positive
On the above bile esculin plate would this be considered a positive or negative result?
Enterococci, Streptococcus bovis
Bile esculin is used for presumptive identification of __________________ and members of the _______________________ ___________________ group.
MacConkey agar
The above plate is yellow before inoculation. What type of agar is this?
Neutral red
What is the indicator in MacConkey agar?
Gram-negative
MacConkey agar inhibits the growth of gram-negative or gram-positive organisms?
Ferment lactose
MacConkey agar is used to isolate and differentiate members of the Enterobacteriaceae based on the ability to _______________________ ____________________ (turns the medium pink).
Coliform
The organism growing on the right side of this MAC plate is a probable _________________ based on the color change and bile precipitate.
EMB, eosin methylene blue
The above plate is inoculates with a gram-negative organism. The organism displays good growth as well as a metallic sheen hue, what is the medium?
Gram-negative
EMB selects for gram-negative of gram-positive organisms?
Coliform
The above organism seen growing on EMB is a probable __________________ based on the amount of growth as well as the metallic sheen color.
Sulfer production, indol, moltility
SIM medium can be used to run three tests. Name these tests:
black precipitate
What would a SIM tube positive for hydrogen sulfide production look like?
Red band on top of medium
What would a SIM tube positive for indole look like?
Entamoeba histolytica
The above organism is a protozoan that belongs to the phylum Sarcomastigophora and the subphyllum Sarcodina. Name this organism
Giardia lamblia
The above image is a protozoan that belongs to the phylum Sarcomastigophora and the subphyllum Mastigophora. Name this organism.
Trichomonas vaginalis
The above image is a protozoan that belongs to the phylum Sacomastigophora and the subphyllum Mastigophora. Name this organism:
Balantidium coli
The above image is a protozoan that belongs to the phylum Ciliophora. Name this organism:
Plasmodium vivax
The above image is a protozoan that belongs to the phylum Apicomplexa. This organism is in it's ring stage. Name this organism:
Plasmodium falciparum
The above image is a protozoan that belongs to the phylum Apicomplexa. Name this organism:
Saccharomyces cerevisiae
The above image is a non-motile fungi. This organism forms unicellular yest cells. Name this organism:
Penicillium notatum
The above image is a filamentous mold. Name this organism:
Aspergillus niger
The above image is a filamentous mold. Name this organism:
Ascaris lumbricoides
The above image is an egg of an organism that belongs to the phylum Nematoda. The organism grown from this egg is a roundworm. Identify what organism will hatch from this egg?
Ascaris lumbricoides
The above image is of an organism that belongs to the phylum Nematoda (rounds worms). Name this organism:
Enterobius vermicularis, pinworm
The above image is of eggs. The organism who these eggs belong to produce many thousands of eggs daily. The organism belongs to the phylum Nematoda (round worms). Name this organism:
Enterobius vermicularis, pinworm
The above image is of multiple organiams that belong to the phylum Nematoda (rounds worms). This organism is very small and reproduces very quickly. Name this organism:
Fasciola hepatica
The above image is of an organism that belongs the the phylum Platyhelminthes (flatworms), class trematodes (flukes). This organism is commonly called a liver fluke. Name this organism:
Schistosoma mansoni
The above image is of an organism that belongs to the phylum Platyhelminthes (flatworms), class trematodes (flukes). This organism's egg has a spike like projection on it. Name this organism:
Schistosoma mansoni
The above image is of an egg that belongs the an organism of the Platyhelminthe (flatworm) phylum, class Trematode (fluke). Name this organism:
Schistosoma japonicum
The above image is of an organism that belongs to the phylum Platyhelminthe (flatworm), class Trematode (fluke). This organism multiplies fastest of the two Schistosomes that were discussed in class. Name this organism:
Taenia solium
The above image is of an organism that belongs to the phylum Platyhelminthes (flatworms), class Cestodes (tapeworms). Name this organism:
Taenia solium
The above image is of an egg from an organism that belongs to the phylum Platyhelminthes (flatworms), class Cestodes (tapeworms). Notice the characteristic hooklets inside the egg. Name this organism:
Taenia solium
The above image is of an organism that belongs to the phylum Platyhelminthes (flatworms), class Cestodes (tapeworms). Notice the scolex and proglottids. Name this organism:
Serratia marcescens
The above organism grows red colonies when grown at the temp of 20-25 degrees. But when grown at 37 degress this organism grows milky white colonies. Name this organism:
Miccrococcus luteus
The above organism grows yellow colonies when grow on TSA regardless of the temperature it is grown at. Name this organism: