Which one of the following would be identified using MSA (mannitol salt agar)
Staphylococcus sp.
Coagulase enzyme causes:
plasma to clot
If gram stained, proteus and enterobacter appear as:
gram - rods
using the IMViC reaction, which genus tests positive for indole and methyl red and negative for VP (acetyl methyl carbonyl) and citrate: (common in polluted water)
escherichia
If enterobacter is present and gives a negative H2S reaction on TSI agar slants what color is the medium
amber
enteric coliform are characterized by all of these except:
gram +
which of these is not a physical means of controlling microbial growth
antibiotics
If a bacterial colony with new characteristics appeared on a plate exposed to ionizing radiation it may be:
mutated
Heavy metal ions interfere with the growth of microbes due to:
oligodynamic activity
Beta hemolysis of blood and positive bacitracin test
s. Pyogenes
which of these is NOT a chemical means of controlling microbes
autoclave
a 'no growth' area around a chemical containing sensi-disc (Kirby-Bauer) is called
zone of inhibition
why can some foods be preserved by salting
dehydrate microbes
which technique is routinely used to determine the degree of contamination of foods and milk
standard plate count
when citrate agar tubes are inoculated with a bacterium that can metabolize this substrate, the medium turns what color after incubation
blue
water may carry all of the following except
lyme disease
which of the is not part of water purity testing
cell wall staining
presumptive, confirmed and completed phases are part of
MPN test
Trichomonas vaginalis is the causative agent of
vaginitis
the mordant for the gram reaction is
iodine
when transferring microbes from tube to tub or tube to plate, the means if sterilizing the loop is
flaming
which of these has the same index of refraction as glass
immersion oil
total magnification working under low power is
100x
which one permits bacterial motility
flagellum
which of these can produce spores (2 answers)
b. cereus and c. tetani
which of these would produce spores for reproduction
aspergillum
penicillin
Rhizopus
all of these
which one grows red pigmented colonies at 25c
s. marcescens
which of these grows metallic green colonies on EMB
e.coli
which of these would not grow preferentially at the top of a broth culture tube
strict anaerobes
which stain technique is used to identify e.coli 0157
gram
all of the following are basic stains except
congo red
serial dilutions were used in making
pour plates
staphylococcus aureus is not characterized by
MSA negative
which broth culture characteristic appears as uniform cloudiness
turbid
the plant body of a mold is called a cyst stage
false
Rhizomes and Aspergillus are examples of Protista
false
Candida albicans may cause lung and vaginal problems
true
when the diameter of the zone of inhibition is largest, the seeded bacterium is probably susceptible to the tested antibiotic
true
plasmodium falciparum is transmitted by a mosquito vector
true
giardiasis lamblia may cause sleeping sickness
false
Yeasts are usually unicellular and often pigmented
true
fungi such as candida and trichophyton may be parasitic
true
protozoan parasites are multicellular
false
Yeasts such as torula may be a food contaminant
true
Cephalosporium mold is a source of antibiotics
true
the red indole ring is extracted by kovac's test
true
starch with clear zones after the use of iodine indicates that amylase is active
true
enterotube tests distinguish among a family of gram positive coliforms
false
e.coli produces spores after 24 hours of growth
false
plasmodium vivid may cause and STD
false
streptococcus lactic shows a gammahemolysis and is found in yogurt
true
EMB plates are both elective and differential
true
cheese products never contain live cultures
false
methyl red is red when acid is present
true
gram negative bacteria appear purple
false
name one food which uses drying technique for prevention
fish
list one heavy mental and one chemical disinfectant that may inhibit bacterial growth
cadmium alcohol
how would you distinguish between an antiseptic and a disinfectant
an antiseptic is applied to the body and a disinfectant is applied to a non-living surface
why is the ELISA test so important in helping us to identify diseases
the acronym ensures that you Assess and Identify for the disease correctly for the proper antibiotic
why are biofilms important? explain and give specific examples of good and bad biofilms
biofilms are important to see how bacteria grows
good: pond water
bad: one on teeth
why are winogradsky columns so useful in studying a variety of aquatic and soil environments
help obtain cultures of a large variety of microbes and help display the growth of new organisms
why is electrophoresis such a helpful tool in identifying various ideas agents
it helps identify and compare different diseases for example DNA strands in gel electrophoresis move to the same location when the DNA is the same
what is the difference between a broad spectrum and a narrow spectrum antibiotic based on observations
broad is more generalized and narrow is considered more specific
what happens if 80 c nutrient agar is poured over plate count culture
it is too hot and will kill the organism needs to be cooled first
why is blood agar such a useful culture medium (give 2 reasons)
see how bacteria affects and grows in blood and more nutrients for growth
an enzyme in staphylococcus that clots plasma
coagulase
in the IMViC reactions e.coli and e.aerogenes show (choose from: same, similar or opposite) reactions
different
list a helpful role of slime molds
help consumption of dead organic materials
give a helpful role for saccharomyces
treating and preventing diarrhea
name a harmful fungus
candidiasis
list 2 positive signs that a sugar has been fermented
growth of yeast and ethanol
why is McConkey's agar considered a selective medium
it helps grow gram negative bacteria
name two helpful protists
Trichonumpha and euglena
what effects does streptococcus progenies have on blood agar
produces a clear hemolysis
name 2 harmful protists
giardia lamblia and plasmodium