Differential Media

The selective agent for MacConkey Agar

bile salts, and crystal violet

indicators for MacConkey Agar

neutral red

Basis for differentation for MacConkey Agar

lactose fermetation

MacConkey Agar turns colorless when:

basic

MacConkey Agar turns red in:

acid

MacConkey Agar inhibits the growth of Gram ____ bacteria

positive

MacConkey Agar supports the growth of Gram ____ bacteria

negaive

MacConkey Agar has ____ numer of sugars

one

The seletive agent for Hektoen Enteric Agar is

bile salts

The indicators for Hektoen Enteric Agar

bromthymol blue, acid fuchsin, and Ferric ammonium citrate

The basis for differentation for Hektoen Enteric Agar

lactose and fructose and salicin fermentation, and hydrogen sulfide production

Hektoen Enteric Agar inhibits the growth of Gram _____ organisms

positive

Hektoen Enteric Agar supports the growth of Gram ____ organisms

negative

Hektoen Enteric Agar is doubley differentail as it has

Sodium Thiosulfate and ferric ammonium

selective agents for Eosin Methylene Blue Agar

Eosin Y and methylene blue dye

Eosin Methylene Blue Agar inhibit the growth of Gram ________ bacteria

positive

The indicators for Eosin Methylene Blue Agar are

eosin Y and methylene blue dye

The basis for differentation for Eosin Methylene Blue Agar is

lactose and sucrose fermentation

Eosin Methylene blue Agar suppors the browth of Gram _______ bacteria

negative

Under _____ conditions, Eosin Methylene Blue Agar produces a dark purple complex usually accompanied by a green metallic sheen.

acidic

A small amont of acid production results in a ____ color for Eosin Methylene Blue Agar

pink

Eosin Methylene Blue Agar is used for the isolation of

fecal coliforms

Columbia CNA with 5% Sheep Blood Agar inhibits growth of Gram:

negative

The selective agents for Columbia CNA with 5% Sheep Blood Agar

colistin and Nalidixic acid

The indicators for Columbia CNA with 5% Sheep Blood Agar

red blood cells

The basis for differentation for Columbia CNA with 5% Sheep Blood Agar

hemolytic reaction

alpha growth

green

beta growth

clears blood

gamma growth

none

Mannitol Salt Agar's selective agents

7.5% NaCl

Mannitol Salt Agar's indicator

phenol red

phenol red turns yellow with a possible hallow in:

acid

Mannitol Salt Agar is pink when pH =

7.4-8.4

Mannitol Salt Agar is red in=

basic

Mannitol Salt Agar basis for differentation

mannitol fermentation

The high sodium cholorid concentration favors the growth of:

staphulococci

This will turn the plate drastic yellow becuase of its ability to ferment mannitol and lower the pH

Staphylococcus aureus