Microbiology chapter 3

Magnetobacterium

microorganisms with an unusual feature: cellular structures called magnetosomes. Prefer areas with little or no oxygen.

Biologists agree that all living things share at least four processes of life

growth, reproduction, responsiveness, and metabolism

Growth

living things can grow; that is, they can increase in size

reproduction

Reproduction means that they increase in number, producing more organisms organized like themselves. Reproduction may be accomplished asexually (alone) or sexually with gametes (sex cells).

Responsiveness

All living things respond to their environment. They have the ability to change themselves in reaction to changing conditions around or within them. Many organisms also have the ability to move toward or away from environmental stimuli�a response called t

Metabolism

Metabolism can be defined as the ability of organisms to take in nutrients from outside themselves and use the nutrients in a series of controlled chemical reactions to provide the energy and structures needed to grow, reproduce, and be responsive. Metabo

Theodor Schwann and Matthias Schneiden

developed the theory that all living things are composed of cells. Cells are living entities, surrounded by a membrane, that are capable of growing, reproducing, responding, and metabolizing.

Prokaryotes

They can make proteins simultaneously to reading their genetic code because a typical prokaryote does not have a membrane surrounding its genetic material (DNA)

Eukaryotes

Have a membrane called a nuclear envelope surrounding their DNA, forming a nucleus, which sets eukaryotes in domain Eukarya.

Organelles

Specialized structures that act like tiny organs to carry on the various functions of the cell.

Glycocalyx

May be composed of polysaccharides, polypeptides, or both

Capsule

When the glycocalyx units of organic chemicals firmly attached to the cell's surface

Flagella

Are long structures that extend beyond the surface of a cell and it's glycocalyx and propel the cell through its environment. (Thin filament, a hook, basal body)

peritrichous

flagella that cover the surface of the cell

spirochetes

spiral-shaped bacteria that have flagella at both ends that spiral tightly around the cell instead of protruding the surrounding medium

endoflagella

form an axial filament that wraps around the cell between its cytoplasmic membrane and an outer membrane.

taxis

movement in response to a stimulus

fimbriae

many bacteria have rodlike proteinaceous extensions. these sticky, bristle like projections adhere to one another and to substances in the environment.

Neisseria gonorrhoeae

causes gonorrhea

biofilms

slimy masses of microbes adhering to a substrate by means of fimbriae and gly cocalyces

pilus

special kind of fimbria . Cells use pili to transfer DNA from one cell to the other via a process termed conjugation.

peptidoglycan

What bacterial cell walls are composed of. (a complex of polysaccharide) is composed of two types of regularly alternating sugar molecules called NAG and NAM, which are structurally similar to glucose. Chains of NAG and NAM are attached to other chains by

Gram-positive

bacterial cell walls have a relatively thick layer of peptidoglycan that also contains unique chemicals called teichoic acids.Some teichoic acids are covalently linked to lipids, forming lipoteichoic acids that anchor the peptidoglycan to the cytoplasmic

Gram-negative

cell walls have only a thin layer of peptidoglycan, but outside this layer is another, outer bilayer membrane composed of two different layers or leaflets. The inner leaflet of the outer membrane is composed of phospholipidsand proteins, but the outer lea

Lipid A

the lipid portion of LPS

Endotoxin

The erroneous idea that lipid A is inside Gram-negative cells. A dead cell releases lipid A when the outer membrane disintegrates, and lipid A may trigger fever, vasodilation, inflammation, shock, and blood clotting in humans.

periplasmic space

Between the cytoplasmic membrane and the outer membrane of Gram-negative bacteria. This includes peptidoglycan and periplasm. Periplasm contains water, nutrients, and substances secreted by the cell, such as digestive enzymes and proteins involved in spec

cytoplasmic membrane

Beneath the glycocalyx and the cell wall. cell membrane or plasma membrane. Composed of phospholipids and associated proteins.

Phospholipid bilayer

A phospholipid molecule is bipolar; that is, the two ends of the molecule are different. The phosphate-containing heads of each phospholipid molecule are hydrophilic; that is, they are attracted to water at the two surfaces of the membrane. The hydrocarbo

fluid mosaic model

mosaic indicates that the membrane proteins are arranged in a way that resembles the tiles in a mosaic, and fluid indicates that the proteins and lipids are free to flow laterally within a membrane.

selectively permeable

It allows some substance to cross it while preventing the crossing of others. Ex a phospholipid bilayer, Large molecules cannot cross through it; ions and molecules with an electrical charge are repelled by it; and hydrophilic substances cannot easily cro

concentration gradient

The difference in concentration of a chemical on the two sides of a membrane

Passive processes

the electrochemical gradient provides the source of energy; the cell does not expend its energy reserve. Passive processes include diffusion, facilitated diffusion, and osmosis.

Diffusion

is the net movement of a chemical down its concentration gradient that is, from an area of higher concentration to an area of lower concentration. It requires no energy out put by the cell, a common feature of all passive processes. For example, oxygen, c

Facilitated Diffusion

Some of these proteins act as channels or carriers to allow certain molecules to diffuse down their concentration gradients into or out of the cell.The cell expends no energy in facilitated diffusion; electrochemical gradients provide all of the energy ne

Osmosis

Water molecules cross from the side of the membrane that contains a higher concentration of water (lower concentration of solute) to the side that contains a lower concentration of water (higher concentration of solute). In osmosis, water moves across the

Isotonic

When solutions on either side of selectively permeable membrane have the same concentration of dilutes, neither side of selectively permeable membrane will experience a net loss or gain of water

Hypertonic

When the concentrations of solutions are unequal, the solution with the higher concentration of solutes is said to be hypertonic. A cell placed in a hypertonic solution will therefore lose water and shrivel (crenation)

Hypotonic

The solution with a lower concentration of solutes is hypotonic in comparison. water will diffuse into a cell placed in a hypotonic solution because the cell has a higher solutes to water concentration.

Active transport

utilizes transmembrane permease proteins; however, the functioning of this transports proteins requires the cell to expend ATP to transport molecules across the membrane.

Uniport

If only one substance is transported at a time

Antiports

Transports two chemicals but in opposite directions

Symports

two substances move together in the same direction across the membrane by means of a single carrier protein.

Coupled transport

For example, H+ moving into a cell down its electrochemical gradient by facilitated diffusion provides energy to carry glucose into the cell, against the glucose gradient. The two processes are linked by a symport. However, cellular energy may still be ut

Group translocation

the substance being actively transported across the membrane is chemically changed during transport. As glucose is transported across the bacterial cell membrane, it is phosphorylated; that is, a phosphate group is added to the glucose. The glucose is cha

Cytoplasm

Cytoplasm is semitransparent, fluid, elastic, and aqueous. It is composed of cytosol, inclusions, ribosomes,and, in many cells, a cytoskeleton. Some bacterial cells produce internal, resistant, dormant forms called endospores.

Cytosol

The liquid portion of the cytoplasm. It is mostly water, but it also contains dissolved and suspended substances, including ions, carbohydrates, proteins (mostly enzymes), lipids, and wastes. The cytosol of prokaryotes also contains the cell's DNA in a re

Inclusions

are often found within bacterial cytosol. Inclusions may include reserve deposits of lipids, starch, or compounds containing nitrogen, phosphate, or sulfur. The presence of specific inclusions is diagnostic for several pathogenic bacteria

polyhydroxybutyrate

polymer of glucose molecules

electrical gradient

Substances that have concentration gradients across cell membranes are electrically charged chemicals. For example, a greater concentration of negatively charged proteins exists inside the membrane, and positively charged sodium ions are more concentrated

gas vesicles

aquatic cyanobacteria contains inclusions that store gases in
protein sacs.

endospores

Which are important for several reasons, including their durability and potential pathogenicity. Endospores are not reproductive structures. Instead, endospores constitute a defensive strategy against hostile or unfavorable conditions. The process of endo

Non membranous organelles in bacteria

ribosome and cytoplasm

Ribosomes

are the sites of protein synthesis in cells. Bacterial
cells have thousands of ribosomes in their cytoplasm, which gives cytoplasm a grainy appearance

Ribosomal RNA

All ribosomes are composed of two subunits, each of
which is composed of polypeptides and molecules of RNA called ribosomal RNA (rRNA)

Cytoskeleton

Which is composed of three or four types of protein fibers. Bacterial cytoskeletons play a variety of roles in the cell. For example, one type of cytoskeleton fiber wraps around the equator of a cell and constricts, dividing the cell into two. Another typ

glycocalyx

That is anchored to its cytoplasmic membrane via covalent bonds to membrane proteins and lipids. The functions of eukaryotic glycocalyces, which are not as structurally organized as prokaryotic capsules, include helping to anchor animal cells to each othe

Cellulose

The walls of plant cells are composed of, a polysaccharide that is familiar to you as paper and dietary fiber.

membrane rafts

Eukaryotic cells use membrane rafts to localize cellular processes, including signaling the inside of the cell, protein sorting, and some kinds of cell movement. Some viruses, including those of AIDS, Ebola, measles, and flu, use membrane rafts to enter h

endocytosis

Which involves physical manipulation of the cytoplasmic membrane around the cytoskeleton, Endocytosis
occurs when the membrane distends to form pseudopods
(false feet) that surround a substance, bringing it
into the cell

Phagocytosis

Eats if a solid is
brought into the cell

pinocytosis

Drinks if only liquid is brought into the cell.

food vesicle

Nutrients brought into a cell by endocytosis

amoeboid action

The cell extends a pseudopod, and then the cytoplasm
streams into it

Exocytosis

Another solely eukaryotic process, is the reverse of endocytosis in that it enables substances to be exported from the cell.

Differences between Eukaryotes and Prokaryotes flagella

Eukaryotic flagella are within the cytoplasmic membrane; they are internal structures that push the cytoplasmic membrane out around them. Their basal bodies are in the cytoplasm. Second, the shaft of a eukaryotic flagellum is composed of molecules of a gl

Cilia

Which extend the surface of the cell and are shorter and more numerous than flagella. No prokaryotic cells have cilia. Like flagella, cilia are composed primarily of tubular microtubules, which are arranged in a "9 + 2" arrangement of pairs in their shaft

Eukaryotic Ribosomes

Within the cytosol of eukaryotic cells are protein-synthesizing
ribosomes that are larger than prokaryotic ribosomes; instead of 70S ribosomes, eukaryotic ribosomes are 80S and are composed of 60S and 40S subunits. In addition to the 80S ribosomes found w

Eukaryotic Cytoskeleton

Composed of an internal scaffolding of fibers and tubules. The eukaryotic cytoskeleton acts to anchor organelles and functions in cytoplasmic streaming and in movement of organelles within the cytosol. Cytoskeletons in some cells enable the cell to contra

centrioles

which lie at right angles to each other near the nucleus

centrosome

in a region of the cytoplasm, plants, algae, and most fungi (and prokaryotes) lack centrioles
but usually have a region of cytoplasm corresponding
to a centrosome. Centrioles are composed of nine triplets of tubular microtubules arranged in a way that res

Nucleus

is usually spherical to ovoid and is often the largest organelle in a cell. Some cells have a single nucleus; others are multinucleate, while still others lose their nuclei. This is often referred to as "the control center of the cell" because it contains

nucleoplasm

the semiliquid matrix of the nucleus

nucleoli

which are specialized regions where RNA is synthesized.

chromatin

which is a threadlike mass of DNA associated with special proteins called histones that play a role in packaging nuclear DNA. During mitosis (nuclear division), chromatin becomes visible as chromosomes

nuclear envelope

Surrounding the nucleus is a double membrane, which is composed of two phospholipid bilayers, for a total of four phospholipid layers.

nuclear pores

Within the The nuclear envelope that function to control the import and export of substances through the envelope.

endoplasmic reticulum (ER)

Continuous with the outer membrane of the nuclear envelope is a netlike arrangement of flattened hollow tubules. The ER traverses the cytoplasm of eukaryotic cells. Endoplasmic reticulum functions as a transport system and is found in two forms: smooth en

Smooth endoplasmic reticulum

plays a role in lipid syn- thesis as well as transport

Rough endoplasmic reticulum

Is rough because ribosomes adhere to its outer surface. Proteins produced by ribosomes on the RER are inserted into the lumen (central canal) of the RER and transported throughout the cell.

Golgi body

is like the "shipping department" of a cell: it receives, processes, and packages large molecules for export from the cell. Not all eukaryotic cells contain this.

secretory vesicles

The Golgi body packages secretions in sacs, which then fuse with the cytoplasmic membrane before dumping their contents outside the cell via exocytosis.

Vesicle and Vacuole

Are general terms for such sacs. Large vacuoles are found in plant and algal cells that store starch, lipids, and other substances in the center of the cell. Often a central vacuole is so large that the rest of the cytoplasm is pressed against the cell wa

Lysosomes

Which are found in animal cells, contain catabolic enzymes that damage the cell if they are released from their packaging into the cytosol. The enzymes are used during the self-destruction of old, damaged, and diseased cells and
to digest nutrients that h

Peroxisomes

Are vesicles derived from ER. They contain oxidase and catalase, which are enzymes that degrade poisonous metabolic wastes (such as free radicals and hydrogen peroxide) resulting from some oxygen-dependent reactions. Peroxisomes are found in all types of

Mitochondria

Are spherical to elongated structures found in most eukaryotic cells. Like nuclei, they have two membranes, each composed of a phospholipid bilayer. The inner membrane forms numerous folds called cristae that increase the inner membrane's surface area. Th

Chloroplasts

Are light-harvesting structures found in photosynthetic eukaryotes. Chloroplasts have two phospholipid bilayer membranes and DNA. The
pigments of chloroplasts gather light energy to produce ATP
and form sugar from carbon dioxide. Numerous membranous
sacs

endosymbiotic theory

The presence of circular DNA, 70S ribosomes, and two bilipid membranes in these semiautonomous organelles, This theory suggests that eukaryotes formed from the union of small aerobic17 prokaryotes with larger anaerobic prokaryotes. The smaller prokaryotes