Game quiz

Which of the following is an endospore producer?

Bacillus subtilis

This staining procedure will only allow you to see the cells and determine the cellular morphology

Simple Stain

What color are the Gram negative cells at the end of the Gram Staining Procedure?

Red

What was used as a mordant in the Gram Stain?

Iodine

Which of the following organisms would be acid fast?

Mycobacterium
Nocardia
Corynebacterium
All of these

Which staining procedure requires the use of Methanol fixation?

Capsular Stain

This procedure must be performed to stain cells that possess mycolic acid in the cell wall.

Acid Fast Stain

Which portion of a dye imparts color?

Chromophore

This staining procedure uses a mordant to enlarge an extracellular structure and make it visible

Flagellar Stain

This staining procedure breaks bacteria into 2 large groups based on the structure of the cell wall.

Gram Stain

This slide prep may be used as an indirect method of observing for motility.

Hanging Drop Slide

Which type of fixation was used in the Gram Stain?

Heat

What structures were green at the end of the endospore stain?

spore

In this staining procedure the cell repels the stain and the background is stained instead.

Negative Stain

This slide prep is ideal for viewing natural, unstained specimens (ex. pond water)

Wet Mount

Inoculation

the transfer and implantation of microbes or infectious material into culture media, or the artificial introduction of a biologic product or pathogenic agent into the body

Culture

the growth of microbes on nutrient medium, or to grow microbes on a nutrient medium

Pure culture

is a culture containing only one species of microbe

Contaminant

is any undesirable microbe that is associated with a culture or other material

Culture Medium

the prepared food material on which microorganisms are grown (cultured)

Colony

a dissociated macroscopic aggregation of a single species of microorganism growing on solid nutrient media.

Aseptic technique

any procedure used in the handling, maintenance, or transfer of microbes that insures the absence of contaminating or infectious microbes

Incubation

is the growth and/or maintenance of microbial cultures in controlled acceptable to optimum conditions.

Incubator

a cabinet used for the incubation of microbes in which the temperature is controlled

Multiple Effect

the total amount of any given substance passed into the environment, by all the cells of a pure culture.