What is normal flora in genital/vaginal/cervical cultures that resembles GC?
Moraxella osloenisis (Moraxilla sp)
found in vaginal specimens, OX +, GN diplococci, but glucose negative (does not ferment glucose)
Capnophilic organisms require
Moraxilla (Branhamella) catarrhalis
grows on nutrient agar at 37*C, is Dnase + and negative for fermentation with all the carbs, CAT and OX +
N. gonorrhoeae is kept best when transported to lab in
When gonorrhea is resistant to standard treatment another drug used is
In recent years gonorrhea has become R to penicillin and is called
Penicillinase producing diplococci
Test used to aid in the detection of N. lactamica is
beta galactosidase test
Test that distinguishes N. lactamica and menigitidis
Thayer-Martin and Martin-Lewis differ from each other due to the inhibitor
Purpose of vancomycin in TM and ML agar is to
Inhibit GP bacteria
Purpose of nystatin and anisomycin in TM and ML agar is to
Modified Thayer-Martin contains
vancomycin, colistin, nystatin, & trimethoprim
N. gonorrhoeae appears as
flat, gray colonies and on GS is gram negative, coffee bean shaped diplococci
When bacteria that you KNOW should be gram negative looks gram positive, what does that say about your GS technique?
Waterhouse-Friderichsen is caused by
Most common serotypes of N. men found in the U.S. are
A, B, C
Which Neisseria tends to autolyse if left in room air too long
Test used to distinguish OX + colonies growing on ML agar & also the Neisseria sp
carb ferm test
All Neisseria sp are OX +
Organism is OX +, GN diplococci, gluc & maltose +. but sucrose & lactose -. What is it?
The CO2 tablet in JEMBEC system is made of
bicarbonate and citric acid
If a urethral smear shows GNC, in pairs with flattened side within the PMNs, how is it reported?
Intracellular GN diplococci
Under what conditions are CTA media incubated?
Non CO2, 35*C for 24 hrs
What is a major cause on nongonococcal urethritis in both males & females?
Form of PID that causes blocked fallopian tubes is?
When you have a small volume of CSF which plate is most important to culture?
95% of meningitis is due to these organisms
H. flu, N. men, S. pneumo
A small GPR that causes meningitis & speiciemia in newborns is
#1 cause of meningitis in newborns is
#2 cause is
GP B strep (S. agalactiae); E. coli
A CSF on a newborn, based on answer to #36 would show what biochemical rxns
CAMP +, bacitracin and bile esculin neg
Epidemic outbreaks of newborn meningitis are often caused by
Elderly, immunocompromised people often have meningitis from (Also causes chronic meningitis)
M. tuberculosis. Also causes chronic meningitis
Aseptic meningitis is caused by what?
Viruses, tumor, drugs, chemicals
What type of meningitis is most associated with CONS and Corynebacterium?
Toxoplasma gondii is a major cause of CNS infections in what population?
Which tube of the CSF collection is used for the cell count?
Which for microbiology?
Crytococcus neofromans in CSF is detected by?
India Ink Test
The Quellung test is used to ID what organisms?
N. men, H. flu, S. pneumo
What pathogens need at lease 10mL of CSF for ID?
Mycobacterium and Crytococcus
If CSF has to be held before culture, at what temp?
If only limited time, which test should be done 1st?
35*C +/-1; Culture and gram stain
Symptoms of meningococcal meningitis?
Stiff neck, rash, high fever
In what age group do the majority of CSF infection occur?