Plasma protein determination can be determined using a(n)
Refractometer
High reticulocyte counts of more than 60,000/mL in dogs and more than 50,000/ml in cats are compatible with
Regenerative anemia
Which of the following statements regarding automated hematology analyzers is not true?
Instrument settings for the size of each cell type are universal to all species
_______________ are important mediators of hypersensitivity reactions and contain substances that can damage and kill some parasites
Eosinophils
Eosinophils
Circulate in low numbers in health
________ is the average amount of hemoglobin in a specific volume of blood and is calculated from the hemoglobin concentration and HCT
MCHC
In some species, neutrophils are called ___________ because of the intense staining of the granules
heterophils
In evaluating a blood smear
the counting area, where most of the evaluation takes place, is between the body and the feathered edge
Another name for nucleated RBCs is
metarubricytes
Fluid collected from throacic and abdominal effusions can be put in EDTA tubes to
prevent clotting in case sample is contaminated with blood
Two tests that can evaluate the intrinsic and common pathways of the coagulation cascade are the activated clotting time (ACT) and the _____________________, which measures the time it takes for the primary platelet plug to form
bleeding time
What are the two pathways of the coagulation cascade?
Intrinsic and extrinsic
Measuring the hematocrit, hemoglobin concentration, and _______________ can be used to determine the oxygen-carrying capacity of blood
RBC count
When collecting blood for a complete blood count (CBC), what statements are true?
If clots are present, a new sample should be collected
2ml of blood is sufficient for a CBC from any animal species
The collection tubes should be inverted 10-15 times before further processing to ensure the RBCs, WBCs, and platelets are evenly dispersed
In a blood smear, oxidative damage results in ________________, which are RBCs in which the hemoglobin has shifted to one side of the cell, creating a clear area outlined by a thin rim of membrane
eccentrocytes
RBCs from ____________ have the largest diameter and the least anisocytosis
dogs
Anisocytosis can be determined using ____________ as a mathematical index
RDW
Unstained smears being sent out to a reference laboratory should be
stored at room temperature
You examine a microhematocrit tube after centrifugation. The buffy coat
contains white blood cells and platelets and is the middle layer between plasma and RBCs
Manual determination of WBC counts can be made using a(n)
PCV card and grid
Red-topped fluid-collection tubes
do not contain EDTA
Hemoglobin is
the protein in RBCs that carries oxygen from the lungs to the tissues
________________ are the predominant type of circulating cell in cattle, sheep, and goats
Lymphocytes
______________ result from antibody binding to the RBC surface and removal of a portion of the membrane by macrophages; this occurs in immune hemolytic anemia, which is most commonly recognized in dogs
Spherocytes
Spherocytes
RBCs that lack a central zone of pallor and often appear slightly smaller and denser than normal RBCs
How do spherocytes form?
Antibody binding to the RBC surface and removal of a portion of the membrane by macrophages in the spleen
A modified Wright or Wright-Giemsa stain
will stain mast cell granules and granules in some lymphocytes purple
A Rouleaux formation
is normal in horses and often shows RBCs appearing like stacked coins
Platelets in mammals
do not have nuclei
Platelets in birds and reptiles
have nuclei and are called thrombocytes