Reagent: Tetramethyl p-phenylenediamine dihydrochloride, Purple (+)
Oxidase
Reagent: Alpha-naphthylamine, sulfanilic acid; Pink (+) w/ zinc powder
Nitrate
Reagent: O-nitrophenyl-B-d-galactopyranoside; Detects: B-d-galactosidase, Yellow (+)
ONPG
Reagent: Media green, Blue (+)
Citrate
Detects urea hydrolyzed to ammonia & CO2, uses phenol red indicator turns pink (+)
Urease
Reagent: KOH & alpha-naphthol, detects acetylmethylcarbinol (acetoin); Pink (+)
Voges-Proskauer
Reagent: Ferric Chloride, Green (+)
Phenylalanine deaminase
Test that measures ability to decarboxylate amino acids:
Lysine ->?
Ornithine ->?
Arginine ->?
Indicator dye ->?
Lysine ->Cadaverine
Ornithine ->Putrescine
Arginine ->Putrescine
Indicator dye ->Bromcresol purple
Lysine Iron agar (LIA)
Indicator?
____% glucose & 1 % _____
(+) result?
Slant of LIA turns______ for lysine deaminase
Indicator? = H2S
0.1 % glucose & 1% LYSINE
(+) result =PURPLE BUTT
Slant of LIA turns RED for lysine deaminase
Motility-Indole-ornithine (MIO)
____% glucose & 1%_______.
Also tests for _____&____.
0.1 % glucose & 1% ORNITHINE
(+) result =PURPLE BUTT
Motility & Indole (Kovac's)
Reagent: (Kovac's)-p-dimethyl-aminobenzaldehyde, Pink (+)
Split from tryptophan
Indole Test