list the equipment found in the phlebotomy lab
1. gloves
2. tourniquet
3. needles
4. tube holder/ needle adapter
5. tubes
6. syringes
7. lancets
8. sharps disposal
9. alcohol pads
10. gauze
11. band-aids
12. pens
13. ammonia salts
14. transport containers
15. phlebotomy trays
16. drawing stains
17. in
are crucial for your safety in phlebotomy
gloves
it is important to make sure your gloves _____
fit
tourniquets are _____ free at UD
latex
is a skill that needs to be mastered in phlebotomy
tying a tourniquet
the measurement of the needle diameter
gauge
the most common gauge for veinpucture
21 or 22
gauge for blood bank donor collection
16 or 18
gauge for very small veins
23
the two different kinds of needle in phlebotomy
syringe and evacuated tube needle
the parts of a syringe needle
point, shaft, hub
the parts of an evacuated tube needle
point, shaft, hub, stopper-puncturing end, sheath
contains a needle shield
portex needle-pro and the BD eclipse
needle that can retract into the safety
vanishpoint
use for difficult blood draws such as the elderly and small children
winged blood collection needles
winged blood collection needles are also known as
butterflies
butterflies can come in a ______ variety
push button
for micro capillary blood draws
blood lancets
blood lancets contain a variety of ______ and types including _____
point length; semiautomatic
term that mean does not allow for clotting
anticoagulant
the clear liquid portion of blood the remains after coagulation occurs
serum
the clear liquid portion from anti coagulated blood that contains platelets and coagulation factors
plasma
pathway that is contained in the blood
intrinsic pathway
pathway that is stimulated by tissue damage
extrinsic pathway
chelate calcium in both the intrinsic and extrinsic pathway
EDTA, sodium citrate, potassium oxalate
chelates thrombin in the common pathway
heparin
lavender, purple or pink stopper
ethylenediaminetetraacetic acid (EDTA)
EDTA prevent blood from clotting by chelating ______
calcium
used for hematology studies
EDTA
why is EDTA used for hematology studies
because it inhibits clumping of cellular components
is now also used for immunohematology in many hospitals
EDTA
immunohematology is also known as
blood banking
how many times should an EDTA tube be inverted
8 times
light blue stopper
sodium citrate
sodium citrate prevents blood from clotting by chelating ______
calcium
is used for coagulation studies
sodium citrate
this tube must be completely filled
sodium citrate
how many times should a sodium citrate tube be inverted
3-4 times
green stopper
heparin
heparin prevent clotting by inhibiting the activity of ______
thrombin
often used in chemistry when plasma is required
heparin
the three different kinds of heparin
sodium, lithium, and ammonia
why would you not use sodium heparin if you want to test the level of sodium electrolytes in a patient
because you would be adding sodium to their blood
how many times does heparin tube need to be inverted
eight times
gray stopper
potassium oxalate
the additive in potassium oxalate tubes
sodium fluoride
what is sodium fluoride role in the potassium oxalate tube
inhibits glycolysis
potassium oxalate inhibit clotting by chelating _____
calcium
used most frequently for glucose analysis when there will be a delay in testing
potassium oxalate
how many times should the potassium oxalate tube be inverted
eight times
red stopper of tiger stopper
no anticoagulant
the anticoagulant tube may have this color top
gold
used for most chemistry, serology, immunology, and immunohematology test that require serum
no anticoagulant
no anticoagulant with a glass tube contains _____
no additives
no anticoagulant with a plastic tube contains _____
clot activators
name the other tube additives
serum separators and clot activators
tube sizes vary from
microcontainer to 10 ml
SST stands for
serum separator tubes
PST stands for
plasma separator tubes
liquifies upon centrifugation and move between RBCs and serum/plasma
thixotropic gel
what happens to thixotropic gel when the centrifuge stops
solidifies and forms a barrier between RBC's and serum/plasma
explain the order of draw
1. sterile specimens for blood culture
2. coagulation study (blue)
3. serum tube with/without clot activator or gel (red/gold)
4. heparin tube (green)
5. EDTA tube (pink)
6. potassium oxalate (gray)
why is the order of draw so important
anti-coagulant carry over
what would be the problem if lavender was collected before marble red
-marble red is a serum tube that need to clot
-EDTA action chelates calcium which is already tested for in marble red
what would be the problem if a tube was collected before blood culture bottle was filled
bottle is no longer sterile