Phlebotomy, Order of the tube and tubes color

order of the tubes
- Sally =
- Brings =
- Really =
- Good =
- Grease =
- Leaves =
- Gravy =

sterile (yellow)
blue ( light blue)
red (glass)
gold (sst)
green
lavander
gray

SST
tiger, marble, covac,
gold, speckled, red/gray
umbrella tube: CMP ( comprehensive metabolic panel)

Additive: silica (glass) no invert
celite (plastic) invert 5-8 time
Function: coagulation
TEST: Total cholesterol, LDL (bad chol) HDL(good)
-NFBG: none fasting blood glucose
-NFBS: none fasting blood sugar
-organ panel
-electrolytes
-drug quantification
-

lavender tube
umbrella tube: CBC ( complele blood count)

CBC count: - RBC erythrocytes
- WBC count: leukocytes
- Platelet count: thrombocytes
- hemoglobin: Hgb, Hb, Hg
- hematocrit: Hct

lavender tube

Additive: EDTA invert 5-8 time
Function: anticoagulation inhibits clot form
Test: RBCs, WBCs
-WBC diff
-Mg: magnesium
-Hct: hematocrit
_HG: A1c, A1c (diabetes)
_ESR: (arthritis)
_sickle cell anemia
Specimen: Whole blood, plasma
Department: Hematology

lavender tubes
WBC differentials count

- Neutrophils = bacteria
- Basophils = allergy \ asthma
_Eosonophils = allergy
-lymphocytes = virus
-monocytes = TB

Tan stopper tube

Additives: EDTA
Function: anticoagulant inhibits clot form
Test: Lead level
specimen: whole blood, plasma
Department: special chemistry, toxicology

light blue tube
umbrella : coagulation

additive: sodium citrate Invert 3-4 time
Function: anticoagulation
test: pt, ptt, aptt, tt, bt, FND,INR
specimen: whole blood plasma
department: coagulation

light blue tube tests

1- Pt = prothrombin time = ( protime ) monitors coumadim ( warfarine, blood thinner
2- Aptt or ptt = Activated partial thromboplastin time , monitor heparin
3-FDP = fibronogen degradation products
4- tt = thrombin time
5- BT = bleeding time
6- INR = inter

gray tube

additive: 1 -sodium fluoride = preserves glucose for 3 days
2 - potassium oxalate
3- lithium - preserves glucose for 24 hrs
Function: preserve glucose
test: FBS = fast blood sugar
-gtt, ogtt = glucose tolerance test
- Blood alcohol level
specimen: whole b

royal blue tube

additive: EDTA
function: anticoagulant
test: trace elements
-toxicology
-nutrition (chemistry depart)
-determination
specimen: whole blood, plasma
department: chemistry
-special chemistry ,toxicology

pink tube

additive: EDTA
function: anticoagulant
teste: blood bank
-testing pregnancy
specimens: whole blood
department: hematology,
blood bank

green tube
umbrella: chemistry

additives: lithium heparin
-ammonium heparin
-sodium heparin
function: anticoagulation (inhibits clot form)
test: ammonia ( must be kept in ice)
-stat electrolytes
_ABG ( arterial blood glucose - keep in ice )
department: chemistry

red ( glass ) tube

-No additive
-No function
test: TDM ( therapeutic drug monitoring )
-RH
-blood type
_HCG
-HIV , ab antibody screening
specimen: serum
department: serology, blood bank

red ( plastic ) tube

additive: silica (glass), celite (plastic)
function: coagulant
tests : total cholesterol
LDL ( bad chol)
HDL(good chol)
NFBG: none fasting blood glucose
NFBS:no one fasting blood sugar
electrolyte
organ panel
drug quantification
trigl

yellow ( sterile)

additive: SPS ( sodium polyanethole sulfonate)
function: anticoagulant
test: blood culture
specimen: whole blood, plasma
department: microbiology

yellow ( non- sterile )

additive: ACD ( acid -citrate dextrose )
function: anticoagulant
test: paternity testing,
-DNA test
specimen: whole blood, plasma
department: chemistry