In Erin Cram's lab, she has a transgenic C. elegant worm expressing the green fluorescent protein (GFP). Expression of GFP is from a promoter regulated by the transcriptional activator End-3. If she injects end-3 double stranded RNA (dsRNA) into that worm
Express lower levels of GFP protein and become less green
All of the following are true about RNA interference (RNAi) except:
It does not affect protein levels
A lac minus mutant (lac -) bacterial mutant
Cannot use lactose
Select the correct statement about a transposon carrying an antibiotic resistance gene
If the transposon 'hops' into a conjugative plasmid, it could rapidly spread to a new bacteria
In the presence of lactose, LacI (the lac repressor):
Does not bind DNA
Hypertrichosis is a recessive X-linked disorder in which an abnormal amount of facial and body hair is produced. Females with hypertrichosis have a patchy pattern of body hair growth. What is a reasonable explanation for this patchy phenotype?
In heterozygotes, random X-chromosome inactivation during embryogenesis produces the patchy skin phenotype.
In a DNA replication fork, the RNA primer
5' CAAGCAACCCAAGUGGGC 3' would be hybridized to which DNA sequence?
3' GTTCGTTGGGTTCACCCG 5'
How are haplotypes transferred to offspring?
Via the Y-chromosome, so only to males.
Main differences between DNA and RNA?
Sugar used in backbone, deoxyribose has one less oxygen
Uracil replaces Thymine in RNA
The transposable element Tn5 includes the following genes: antibiotic resistant genes neo-r, etc, and gene IS50R that encodes transposase. Which of the following would be likely to enhance the spread of antibiotic resistance?
The transposon inserts itself into F factor.
Which of the following features is common to both DNA replication and transcription?
A sugar-phosphate bond is formed between 3' hydroxyl group and 5' phosphate
Transcription factor PAX-6 binds to enhancer region of 5' of PAX-6 gene and increases transcription of pax-6 mRNA. This is an example of
Positive feedback
Transcription terminates when:
Release factor moves into the A site, polypeptide is released.
Select the correct statement regarding PCR:
It is used to create many copies of a specific DNA sequence
What is a peptide bond?
A bond between amino acids (On test, is the C-N bond)
The process of ______________ eventually results in different versions of the same protein being produced
Alternative splicing
The operon "Z" in bacteria contains two genes, a and b. These genes code for the proteins A and B, which allow the bacteria to consume sugar "zaltose". Another protein, protein R, is a repressor for expression of operon Z. Which of the proposed regulatory
When zaltose binds protein R, protein R no longer represses operon Z. (Similar to lac operon)
A child is born to a couple, and the chariot of the child shows he is 47, XXY. What could explain this finding?
Non-disjunction in any of the following:
Father during Meiosis I
Mother during Meiosis I or II