JMU BIO 270 LAB PRACTICAL EXAM

bar graph

discrete categories

independent variable

control; x-axis

dependent variable

y-axis, measured

Caption of graphs

Figure 1. "The effect of" __ on __.

1 Tailed

is the mean larger, smaller than the other

2 Tailed

is there a difference between the two

paired

same person being tested ( before and after comparison)

unpaired

separate independent groups

P> .05

there is no significant difference

P<.05

there is a significant difference

Scatter plot

continuous numbers

trendline

polynomial or linear ( which ever fits the graph best just have to see)

Membrane Transport Lab

Waters is permeable to the bag of glucose and it moves into the bag and up the tube because of osmotic pressure and because of hydrostatic pressure the osmosis=diffusion

Osmotic Pressure

pulls the water and glucose up the tube

Hydrostatic Pressure

pushing down like gravity and stops the movement of sugar solution

Membrane is permeable to solute

solute moves from high to low concentrations

Membrane is impermeable to solute

H2O moves from high to low concentrations
H2O moves from low to high solute concentrations

Diffusion rates are different because...

Fick's law: molecular weight is inversely related to diffusion rate

Isotonic

cloudy RBC

Hypertonic

water moves from inside to outside
Crenation (shrivels up)
cloudy RBC

Hypotonic

water moves from outside to inside
Hemolysis (swells) and bursts
clear

Enzyme concentration

indicates inhibitor; increasing enzyme concentration= increases reaction rate; linear (noncompetitive fashion)

Substrate concentration

indicated saturations; increasing substrate= increases rate until plateau at x=__

pH graphs enzymes

optimal point at highest and denaturation at low and highest pH

Temperature graph enzymes

optimal point highest and denaturation high temp

inhibitor

noncompetitive- allosteric
competitive- active site
increase inhibitor decreases rate because unable to bind to enzyme

Ionic Concentration

...

Metabolism: as size increases

SA increases= SA:V ration decreases
Volume increase= Metabolic rate decreases
O2 intake increases= body heat escapes more slowly

Nomogram

graphical tool to determine SA using weight and heigh

Reflex arc steps:

1. receptor
2. sensory afferent neuron
3. integration center
4. motor efferent neuron
5. effector

Reflex

single synapse on spinal cord
monosynaptic
shorter latency period

Reaction

multiple synapse with CNS
polysynaptic
longer latency period

Number is synapses involved

In reflex fewer then reaction
In reaction greater then reflex

Find latency period on graph

M on beginning of Stimulus (top graph)
X on beginning of angle (Bottom graph)
all in seconds,

Find Angle of rotation on graph

M on beginning of angle
X on peak of angle
Magnitude (degrees)

Adaptation

occurs sensory receptors change their sensitivity to the stimulus
Ex: fingers in water

Phasic receptors

rapid adapt; cold pool,

Tonic receptors

takes awhile to adjust

Referred Pain

stimulation of one part of the body gives rise to sensations that are seen localized in different parts of the body
Ex: elbow and pinky and heart attacks

Dermatome

referred pains areas on skin that supply nerves from a single spinal root; "false pain

Air conduction

normal way of hearing

Boone conduction

cranial bones directly to cochlea

if patient can't hear through air conduction but CAN through bone conduction:

problem exists through outer or middle ear (inflammation)

if patient can't hear through air or bone:

problem exists within the inner ear (cochlear damage)

Rods

non-color vision; discriminative
greater perceptive field

Cones

color
smaller receptive fields

Receptive fields

How many points felt tells how many receptive fields there are, more sensitive area has multiple receptive fields ( so you would only feel one sensation), less sensitive more spread out so you would feel both spots.
1 felt= 1 receptive fields
2 felt= 2 re

Taste

need saliva to dissolve sugars to be able to taste; with a dry tongue you cannot taste

Temporal summation

increasing the number of times stimulus occurs every second one at a time
increasing frequency
leads to tetany
sequential

Motor Unit recruitment

increasing stimulus will increase the number of motor units
eventually maximum of motor units will be stimulated
instantaneous

Spatial recruitment

multiple motor units are recruited at the same time to increase the force
increase the voltage

Frequency of a twitch

used to increase or decrease multiple twitches

Voltage of a twitch

used to increase or decrease a single twitch

Finding force of a single twitch on graph

M at bottom of force
X at peak of force
in FU units

For a single twitch...

the contraction time is shorter than relaxation time

To calculate the % in strength go muscle contraction:

largest peak- smallest peak / smallest peak x 100

To calculate the frequency of stimulation:

1/ i x 1000 where the i is the interval given from the graph or you will find from bottoms of the two peaks
(UNITS: Stimulus/seconds)

R-R value is found by:

tops of the two peaks of QRS's
seconds

Heart rate:

1/ R-R x 60 = beats/mins

Pulse wave velocity:

length of arm / M at bottom of S wave and X at the start of the pulse wave
(UNITS:meters/second )
Normal is between 4 to 9

Atrial depolarization

P wave

Ventricular depolarization

QRS wave

Ventricular repolarization

T wave

Systolic pressure

first sound, top number, contracts

Diastolic pressure

second sound, bottom number, rests between beats, refilling

Pulse Pressure=

systolic - diastolic

MAP= mean arterial pressure :

diastolic + 1/3 pulse pressure

Korotkoff sounds

heard by sphygmomanometer between systolic and diastolic pressures

Vital capacity

TV+IRV+ERV

Respiration rate

60 sec/ i where i is the interval time between each normal breath
breath/min

IRV

peak of normal breath to peak of big breath

Tidal Volume

one normal breath
peak of normal breath to bottom of normal breath

ERV

bottom of normal breath and the bottom of the big breath

Spirometer

instrument used to produce the above lung volumes

Total lung capacity(TLC)

TV+IRV+ERV+RV

what happens to TV and IRV after exercising

the TV increases and IRV decreases b/c the VC stays the same

Obstructed lung disease

<60%; decreases ability to empty lungs(exhale)
asthma and emphysema

Restrictive lung disease

>90% decreased ability to fill lungs(inhale)
pulmonary fibrosis

Normal breath %

80%

Breath-hold time

decreases after exercise(increases CO2) and increases after hyperventilation (decreases CO2)

Protein in urine

too large to be filter at glomerulus

Glucose in urine

reabsorbed in bloodstream, usually because sugar is in the blood and related to diabetes because insufficient insulin

Ketones in urine

inadequate levels of carbs and patients with diabetes

Hemoglobin

usually with women who are menstruating

Specific Gravity

use refractor to measure, look where colors line up
between 1.001-1.03 is normal

Higher Specific gravity

greater solute to water ration
yellow, dark yellow
1.02-1.03
dehydration

Lower Specific gravity

greater water solute ration
diluted, clear, pale yellow
1.002-1.008: closer to 1 which is water
hydrated

Carb digestion

mouth

Protein digestion

stomach

lipid digestion

duodenum

Carb/protein/fat absorption

jejunum

gall bladder

secretes bile, acids breakdown of triglycerides, lips, and fat to amino acids

pancreas

secretes pancreatic enzymes that break down carbs, proteins, and fats

peristalsis

a series of wave-like muscle contractions that moves food to different processing stations in the digestive tract. the process begins in the esophagus when a bolus of food is swallowed

Mouth

Digested: Start- Starch
Absorbed: nothing

Esophagus

Digested: starch
Absorbed: nothing

Stomach

Digested: starch and the start if protein
Absorbed: nothing

Duodenum

For a normal meal and one with added dietary fiber: (Digested starch, Protein, and lipid start, Absorbed: nothing)
For a person with pancreatic insufficiency: (Digested and absorbed nothing)
If Gallbladder Disease: ( digested; starch and Protein, Absorbed

Jejunum
***Main place for Absorption

Normal mean and meal with DF= (Digested: Finish starch, protein, lipid. Absorbed:mono/disaccharides, amino acids, fatty acids)
Pancreatic: ( nothing digested or absorbed)
Gallbladder: ( Digested: finish: starch and protein, Absorbed: mono/disaccharides. A

ileum

Digested: nothing
Absorbed: Bile Salts

Large Intestine

Digested: nothing
Absorbed: Water

Waste Elimination

Normal: nothing
DF: Fiber, water, toxins
P: starch, proteins, lipids
G: lipids