LADY MACBETH: "[...] unsex me here and fill me from the toe-top full of the direst cruelty
Lady Macbeth calls upon the spirits to make her more masculine and strong to carry out the deed of killing Duncan
MACBETH: "Prithee, peace:I dare do all that may become a man".
This is when Lady Macbeth tells him he looks green and pale, which during the time was signs of anemia, seen to be a disease typically for young, virgin girls. He replies saying he is a man.
MACDUFF: "O gentle lady, 'Tis not for you to hear what I can speak.The repetition in a woman's earWould murder as it fell.
Macduff tells Lady Macbeth that she is too gentle to even hear about a murder. However, the irony is that she in fact plotted the murder.
LADY MACBETH: "Are you a man?
Lady Macbeth once again questions his masculinity when he claims to see the ghost of Banquo at the dinner table.