What are the different types of light microscopy?
Bright fieldDark fieldPhase contrastDifferential interferenceFluorescenceConfocalTwo photon excitation
What are the different types of electron microscopy?
Transmission EMScanning EM
What is the typical size of a red blood cell?
7 - 8 micrometers
What is the typical size of an eukaryotic cell?
15- 50 micrometers
Which type of microscopy has the highest resolution?
TEM
What are common fixatives for light microscopy?
Glutaraldehyde - cross links proteins at amino groups and carbohydrates on hydroxyl groups
What are common fixatives for EM?
Osmium tetraoxide - cross links double bonds. commonly used to fix lipids in membrane Cryofixation - sample is rapidly frozen in liquid ethane
What are common stains for light microscopy?
Hematoxylin and Eosin. Hematoxylin is a (+) dye so it stains negative molecules like nucelic acids, glycosaminoglycans, and ribosomesEosin is a (-) dye so it stains positive molecules like extracellular fibers, and cytoplasmic filaments
Which dye stains lipids?
Sudan black
Which dye stains reticular fibers?
Reticular fibers (spleen and lymph nodes)
What are common stains for transmission EM?
Staining employs deposition of heavy-metal based compounds.Osmium tetroxide, uranyl acetate, lead citrate
What kind of microscopy lets you see living cells?
Phase contrastDifferential interference
What type of microscopy is usually used for thicker specimen?
Confocal microscopyTwo - photon microscopy
What kind of stain/coat does negative staining involve?
Uranyl acetate
What can you see with freeze fracture techniques?
Internal membrane structuresex: inside of extracellular layer vs inside of cytoplasmic layer