Q1 Biochem Exam 2 Content

function of carbs

E storage, cell mem comp, intercellular communication, struct support

empirical formulasmallest sugar

CH2O3 C (triose)

classifications of carbs

monosacc: simple/reducingdisacc: form via glycosidic bondsoligosacc: 3-10 subunitspolysacc: 10+ to 100 subunits (indigestible)

how do sugars go from linear to cyclic

C5 binds to C1

fructose: linear to cyclic

6 C chain forms 5 member ringC#1 is sticking off of anomeric C(#2)

oral tolerance test

test for abnormal digestion AKA hormone test(high ketone in blood means high acetone in breath)

catabolic vs anabolic

break downbuild up

catabolic and anabolic rxns are regulated by ?

intracellular communication (avaialability of subst and enz activity/inactivity

ATP - 2 P gps =

cAMP

kinase vs lyase

adds P gpremoves P gp

GLUT 1-14 requires E: T/F

false

? uses E to move glucose against gradient

Na dependent glucose transporters(SGLT)

does glucokinase of hexokinase have a higher Km

glucokinase

what does adenylyl cyclase do

ATP to cAMP

where does glycolysis occur

cytoplasm of all tissue cells

what is req for glycolysiswhat is prod in glycolysis

2 ATP, 2 NAD+4 ATP, 2 NADH

where does gluconeogenesis occur

liver and kidneys

how many rxns in glycolysis vs gluconeogenesis

1011

how long is glycogen stores in liver for

10-18 hours

first two steps of carb dig

1: salivary amylase in mouth2: pancreatic amylase in SI

what bonds are found in glycogen vs cellulose

a1-4B1-4

how does glycogen get from the lumen to the outside

1: Na inside moves out via Na channels (this makes E)2: this E is used to move the glucose out of the lumen into the blood3: Na/K pump returns Na and K to normal

? increases when glucose gets too high in bloodthis leads to ?

insulinglycolysis

GLUT 1: Km, location

3everywhere

GLUT 2: Km, location

17liver and pancreas

GLUT 3: Km, location

1.4brain and testes

GLUT 4: Km, location, function

5muscle, fat, heartresponds to insulin signal and allows glucose back inside cell

GLUT 5: Km, location

6intestine, kidneys

glycolysis vs GNG: anabolic vs catabolic

G: CGNG: A

under aerobic conditions, how does glycolysis continue

1: pyruvate combines w lactate dehydrogenase2: pyruvate takes H from NADH to make NAD+3: prod= lactic A

hexokinase does what

adds P to keep it inside cell due to polar membrane

low Km = high or low affinity for glucose

HIGH

positive regulation of glucose

high insulinlow adenyl cyclasePFK-2 dephosp (activates)F26BP+ feedback loop

negative regulation for glucose

high conc: citrate, G6P, glucagon Hhigh adenyl cyclasecAMPprot kinase APFK-2 phosphorylated (deactivate)low F26BP- feedback loop

how is GNG regulated

opposites of glycolysis regulation