RifampicinRifamycin
Inhibits initiation of RNA synthesisBlocks formation of first phosphodiester bond
Actinomycin D
Inhibits RNA synthesis by incalating DNACauses mutationsWorks in both pro- and eukaryotes
Alpha-amanitin
Inihibits eukaryotic RNA polymeraseRNA pol I - (nucleolus) insensitiveRNA pol II - (mRNA) highly sensitiveRNA pol III - (tRNA) moderately sensitive
Antibiotics to bacteria
Inhibit protein translationAct of rRNA, not the ribosomal protein
Diphtheria toxin
Inihibits translocation of protein being synthesized
Puromycin
Causes premature chain terminationActs like aminoacyl-tRNAAffects both pro- and eukaryotes
Streptomycin
Inhibits initiation and causes misreading of mRNA in prokaryotes