Ochem Lab Column Chromatography


Mobile component of Column Chromatography


Organic Solvent


Stationary component of Column Chromatography


Solid absorbant


Solid support component of Column Chromatography


The column


Physical Property component of Column Chromatography


Polarity


Detection component of Column Chromatography


Color (UV light also)


Pavilion coefficient


kp(x) [x] stat/ [x] mobile


Compounds with a higher affinity for the stationary phase will...


Will be retained longer


Stationary phase


Typically POLAR material


Examples of stationary phase


Polar material

Silica Gel (SiO2) : Slightly aidic...avoid use with acid sensitive compounds...basic tend to have a larger affinity

Alumina (NaAl2O3): Slightly basic

Silicon Gel (C18): Less common reverse phase..nonpolar instead of polar


Mobile phase


Organic solvents-Can cahnge the polarity of mobile phase through a gradient -Mix nonpolar and polar solvents; polarity
-Changes with

-Solvents must by misuble


Examples of Mobile Phase


Nonpolar: Hexanes

Polar: Acetone