Cover the smear with crystal violet for 20 second
Gram staining
What is the mordent for gram staining
Gram's iodine
Decolorize the stain with 95 percent ethnal alcohol
Gram's staining
Cover the stain with safranin for 20 second
Gram staining
In gram staining bacillus cereus what color do rods stain
Blue
Is bacillus cereus gram pos or Neg
Pos
Is escherichia coli gram pos or gram neg
Neg
Is staphylococcus aureus gram pos or neg
Pos
In negative staining what stain is used
Nigrosine
Do you heat fix a negative stain
No
Place the edge of the clear slide into the nigrosine by backing it
into the nigrosine and dragging it foward.Negative staining
Sarcina lutea negatve stain results
4 cocci, tefrad cellular arrangment
Bacillus cereus negative stain results are long rods
Cover the stain with 1percent aqueous solution of crystal violet
Capsual staining
Wash with 20percent copper sulfate
Capsual staining
Do you heat fix a capsular stain
No
Streptococcus lactis capsular stain results
Two nucleus in one cell (like an egg)
Enterobacter aerogenes
Rods longer than ecoli
What is the primary stain used for acid-fast staining
Carbol fuchsin
What is the mordent for acid fast staining
Steam heat
What is the decolorizer for acid fast staining
95percent ethenol alcohol
What is the counter stain for acid fast staining
Methylene blue
What are the results of mycobacterium smegmatis with a acid fast
stainingRed rods Blue cocci
What is the primary stain in spore staining malchite green
Cover the slide with malachite green a place slide atop a beaker of
boiling waterSpore staining
Counter stain the stain with safranin
Spore staining
What is the counter stain in spore staining
What is the result of spore staining escerichia coli and bacillus cereus
Red rods and green spores
To isolate a specific bacteria what method is used
Pure culture
Name two types of prue culture methods
Streak plate method and Pour plate method
What are the three types of gaseous requirements
Aerobic, anaerobic and facultative
what three methods are used to find the gaseous requirment of a
bacteriaWright tube, thioglyollate and Petrie plate
Slightly loosen the lid of a tube of thiogycollate broth and boil it
for 10 minutes, allowing the dissolved oxygen to be driven off.Thioglycollate broth method
Obtain a nutrient agar slant tubes, inoculate it with bacteria, place
a cotton plug into the test tube about one- fourth inch from the top of the slantWright's tube method
What acid is uesed in the wright's method
Pytogallic acid
What base is used in the wright's method
10 percent NaOH
Sterilize an inoculating loop and asepically streak one loopful of
bacteria onto one side of a Petri platePetri plate method
What is the gaseous requirment of escherichia coli
Facultative
What is the gaseous requirment of clostridium sporogenes
Anaerobic
What is the gaseous requirment of sarcina lutea
Aerobic
What is the gaseous requirment of bacillus subtilis
Aerobic
Does escherichia coli grow in thioglycollate broth method
Yes
Does escherichia coli grow in
The wright's tube methodYes
Does escherichia coli grow in
Petri plate methodYes
Does clostridium sporogenes grow in thioglycollate broth method
Yes
Does clostridium sporogenes grow in
Wright's tube methodYes
Does clostridium sporogenes grow in petri plate method
No
Does sarcina lutea grow in triogycollate broth method
No
Does sarcina lutea grow in wright's method
No
Does sarcina lutea grow in petri plate method
Yes
Does bacillus subtilis grow in the thioglycollate broth method
No
Does bacillus subtilis grow in the wright's tube method
No
Does bacillus subtilis grow in the petri plate method
Yes
What are the cultural characteristics of bacillus cereus
Flat, white, dry, motile, billowy, opaque
What are the cultural characteristics of escherichia coli
Raised, white, mucoid, motile, translucent
What are the cultural characteristics of proteus vulgaris
Flat, white, mucoid, motile, translucent
What are the cultural characteristics of staphylococcus aureus
Raised, white, mucoid, not motile, opaque
What is hanging drop slide method used for
To study motility
What is the motility test agar method used for
To test for motility
What are the results of motility test on proteus vulgaris
Motile
What are the results of motility test on pseudomonas aeruginosa
Motile
What are the results of motility test on escherichia coli
Motile
What are the results of motility test on sarcina lutea
Not motile
In sugar fermentation the results of dextrose and bacillus subtillis
Acid/no gas
In sugar fermentation the results of dextrose and escherichia coli
Acid/gas
In sugar fermentation the results of dextrose and staphylococcus aureus
Acid/no gas
In sugar fermentation the results of dextrose and alcaligenes
viscolactisBase/no gas
In sugar fermentation the results of lactose and bacillus subtillis
Base/no gas
In sugar fermentation the results of lactose and escherichia coli
Acid/gas
In sugar fermentation the results of lactose and staghylococcus aureus
Base/no gass
In sugar fermentation the results of lactose and alcaligenes viscolactis
Base/no gas
In sugar fermentation the results of maltose and bacillus sbtilis
Base/no gas
In sugar fermentation the results of maltose and escherichia coli
Acid/gas
In sugar fermentation the results of maltose and staphylococcus aureus
Base/no gas
In sugar fermentation the results of maltose and alcaligenes visclactis
Base/no gase
In sugar fermentation the results of saccharose and bacillus subtillis
Acid/no gas
In sugar fermentation the results of saccharose and escherichia coli
Base/no gas
In sugar fermentation the results of saccharose and staphylococcus
aureusAcid/no gas
In sugar fermentation the results of saccharose and alcaligenes
viscolactisBase/no gas
In sugar fermentation the results of sucrose and bacillus subtilis
Acid/no gas
In sugar fermentation the results of sucrose and escherichia coli
Base/no gas
In sugar fermentation the results of sucrose and staphylococcus aureus
Base/no gas
In sugar fermentation the results of sucrose and alcaligenes viscolactis
Base/no gas
What is the reagent in the starch hydrolysis method
Iodine
What are the results of bacillus subtilis in the starch hydrolysis
methodPositive for starch hydrolace
What are the results of staphylococcus aureus in the starch hydrolysis
methodNegative for starch hydrolysis
What are the results bacillus subtilis in the urea hydrolysis method
Negative no urease
What are the results escherichia coli in the urea hydrolysis method
Negative no urease
What are the results proteus vulgaris in the urea hydrolysis method
Positive produces urease
What are the results staphylococcus aureus in the urea hydrolysis method
Negative no urease
The purpose of this exercise is to contrast this enzymatic ability
between various bacteriaLitmus milk method
What color is the base in the litmus milk method
Blue
What color is the acid in the litmus milk method
Red
What are the results of escherichia coli
In the litmus milk methodTurned pink, positive for enzyme lactase
What are the results of alcaligenes viscolactis In the litmus milk
methodNegative for enzyme lactase blue (produces ropey curd)
What are the results of streptococcus lactis In the litmus milk method
Positive for enzyme lactace (pink ring) round top (white clot bottom)
What are the results of proteus vulgaris In the litmus milk method
Negative for lactace (stayed blue) negative ropey curd
What are the effects of catalase on staphylococcus aureus
Positive
What are the effects of catalase on saccharomyces cerevisiae
Positive
What are the effects of catalase on eschericia Coli
What are the effects of catalase on streptococcus lactis
What are the effects of hydrogen sulfide on proteus vulgaris
Positive for Sistine desulerace (enzyme)-enzyme, dark parcipitant (lead sulfide)-product
What are the effects of hydrogen sulfide on eschericia coli
Negative for Sistine desulferase
What do you add to nitrate broth when converting from nitrates to
nitritesSulfanilic acid and dimethyl alpha-napthalamine
What is nitrate reduction
The enzymatic ability to convert nitrates to nitrites between various bacteria
What is the effect of nitrate reduction on proteus vulgaris
Positive for nitratase-enzyme
What is the effect of nitrate reduction on eschericia coli
Positive for nitratase
What is the effect of nitrate reduction on pseudomonas aeruginosa
Positive for nitratase w/zinc
What is the effect of nitrate reduction on staphylococcus aureus
Positive for nitratase w/zinc