MicroLab Midterm

Congo Red Capsule Stain

Differential stain used to visualize capsules
-Uses 2 stains
1.) Congo Red I- acidic stain
2.) Congo Red II- basic stain

Cong Red Capsule Stain Procedure

1.) Put drop of Congo Red I at 1 end of slide and aseptically transfer bacteria to drop using loop.
2.) Using 2nd slide as spreader, pull drop with microbe across slide. Let air dry
3.) Add Congo Red II to slide, wait 1-2 min. then ver gently wash excess