Most sensitive method for measuring low molecular antigens such as IL-2.
Sandwich ELISA
Cells can be tagged with fluorescent monoclonal antibodies for separation by this method.
FACS
Two different enzymes conjugated to the secondary antibody in ELISAs
Alkaline Phosphatase and Peroxidase
Cell size in flow cytometry is measured by this.
Forward Light Scatter
The number of wells in a typical Ouchterlony plate?
7
This method can detect different epitopes on the same antigen.
Ouchterlony
Which method of measuring antibody titers is most sensitive.
ELISA
Antigens can be coated onto red blood cells in this type of assay
Agglutination
3 blockers used in ELISA
BSA, Milk & Tween 20
The matrix used for electrophoresis in western blot
acrylamide
Antibody may be incorporated directly into agarose in which 2 methods?
Mancini & oudin
Avidin binds to this
Biotin
Maximum precipitation occurs at this point where the concentrations of Ab and Ag are optimal
Equivalence
If too much antibody is present for precipitation it is called?
Zone of antibody excess
Best method for antigen separation?
Western Blot
Agent added to samples to allow protein separation only by molecular weight in Western Blots.
SDS
How are antigens transferred from gel to membrane in Western Blots?
Electrophoresis
Two methods involving antigen separation prior to contact with antibody?
Immunoelectrophoresis and Western Blot
PAGE is called reduced when this agent is added to samples.
mercaptoethanol